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BROAD-SPECTRUM METHOD FOR DETERMINING E. COLI FLAGELLAR ANTIGEN TYPE USING PCR METHOD NEW

外国特許コード F180009494
整理番号 S2016-0931-C0
掲載日 2018年11月1日
出願国 世界知的所有権機関(WIPO)
国際出願番号 2017JP025817
国際公開番号 WO 2018016451
国際出願日 平成29年7月14日(2017.7.14)
国際公開日 平成30年1月25日(2018.1.25)
優先権データ
  • 特願2016-144861 (2016.7.22) JP
発明の名称 (英語) BROAD-SPECTRUM METHOD FOR DETERMINING E. COLI FLAGELLAR ANTIGEN TYPE USING PCR METHOD NEW
発明の概要(英語) [Problem] To provide a practical determination method whereby H type determination can be rapidly and easily performed.
[Solution] An Escherichia coli H typing determination method characterized by comprising determining H typing by performing nucleic acid amplification of a polymorphic region of a gene that codes for flagellin constituting flagella of E. coli. Specifically, through the present invention, the H type of E. coli can be genetically determined rapidly and accurately, and it is therefore possible to more appropriately and rapidly prevent infection with or infectious spread of enteropathogenic E. coli including enterohemorrhaghic E. coli in medical or public health/food sanitation settings.
従来技術、競合技術の概要(英語) BACKGROUND ART
E. coli infection by pathogenic bacteria as well as the case occurs, the path a source of contamination and contamination, contamination as much as possible and quickly identified, it is necessary to prevent expansion of the infection.For this purpose, the characteristics of the isolates and correctly determined, the relationship between the strains needs to be clarified.
E. coli in the fine, another O-H based on a type and type-specific sera (O: H serum type) is, as a method for international gold standard are used for a long time.And another O-type, the E. coli bacteria present on the surface of the sugar chain structure as a method of classification as an antigen.In addition, H is a type, the motion of the organ as the flagellar antigen E. coli as a method of classification.The current, 'World Health Organization E. coli for study center in cooperation with a reference' Danish National Laboratory in serum (SSI: by Statens Serum Institut), as the fine E. coli, two types of O of the group 185 (O1-O188, subtype 3 type: O18ab/O18ac,O28ab/O28ac,O112ab,O112ac include, the type 6: O31,O47,O67,O72,O94,O122 is a vacant) and the 53 types of H-type (H1-H56,3 type: H13, H22, H50 is a vacant) is determined (non-patent document 1).
E. coli infection in the present state of the country, a serious cause severe (about 4,000 lines per year) is E. coli, Institute National Institute of infection by a local, substantially all of the isolates serotype determination is performed.However, the reagent is an anti-serum domestic manufacturer sells, corresponding to a part of not only serotype (O of the group: 50 type, H-type: type 22), and the determination cannot be made part of the separation lines has been a problem.A group of inventors, in order to solve this problem, almost all of the group of O can be determined genetically (E. coli O-genotyping PCR PCR method: ECOG-PCR) is developed and, in actual use in the inspection site and Patent Application are reached (Patent Document 1, Non-Patent Document 2).
However, O is determined by only a group of serum, it is not necessarily sufficient. For example, intestinal bleeding as E. coli is widely known in Japan and O157 is, as the name implies, in E. coli O157, Escherichia coli O157 in H7 is found in addition to the serious, pathogenic E. coli H45 or H39 of the gastrointestinal tract, such as a non-pathogenic E. coli H16, a plurality of H-type is known to be included.In addition, a serious national report a level higher than the number of Escherichia coli O103 is always separated, H2, H11, H25 such as H-type is included in the plurality of types.From these, as well as serum group O, H type can also be manufactured, another determination is performed by a precise mold is required.
  • 出願人(英語)
  • ※2012年7月以前掲載分については米国以外のすべての指定国
  • UNIVERSITY OF MIYAZAKI
  • 発明者(英語)
  • IGUCHI ATSUSHI
  • BANJOU MASAYA
国際特許分類(IPC)
指定国 National States: AE AG AL AM AO AT AU AZ BA BB BG BH BN BR BW BY BZ CA CH CL CN CO CR CU CZ DE DJ DK DM DO DZ EC EE EG ES FI GB GD GE GH GM GT HN HR HU ID IL IN IR IS JO JP KE KG KH KN KP KR KW KZ LA LC LK LR LS LU LY MA MD ME MG MK MN MW MX MY MZ NA NG NI NO NZ OM PA PE PG PH PL PT QA RO RS RU RW SA SC SD SE SG SK SL SM ST SV SY TH TJ TM TN TR TT TZ UA UG US UZ VC VN ZA ZM ZW
ARIPO: BW GH GM KE LR LS MW MZ NA RW SD SL SZ TZ UG ZM ZW
EAPO: AM AZ BY KG KZ RU TJ TM
EPO: AL AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HR HU IE IS IT LT LU LV MC MK MT NL NO PL PT RO RS SE SI SK SM TR
OAPI: BF BJ CF CG CI CM GA GN GQ GW KM ML MR NE SN ST TD TG

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