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TECHNOLOGY FOR SELECTIVE PROTEIN SECRETION IN FUNGUS NEW

外国特許コード F180009503
整理番号 (S2017-0213-N0)
掲載日 2018年11月1日
出願国 世界知的所有権機関(WIPO)
国際出願番号 2017JP046107
国際公開番号 WO 2018123856
国際出願日 平成29年12月22日(2017.12.22)
国際公開日 平成30年7月5日(2018.7.5)
優先権データ
  • 特願2016-252330 (2016.12.27) JP
発明の名称 (英語) TECHNOLOGY FOR SELECTIVE PROTEIN SECRETION IN FUNGUS NEW
発明の概要(英語) The purpose of the present invention is to provide: a method for easily obtaining a culture supernatant having a reduced amount of unwanted enzymes; a microorganism that can be used in said method; and a method for producing a target substance using said microorganism. The present invention relates to a modified fungus that expresses a fusion polynucleotide in which a polynucleotide coding for a GPI-added signal sequence is joined to a polynucleotide coding for an endogenously secreted enzyme, and also relates to a method for producing a target substance using said modified fungus.
従来技術、競合技術の概要(英語) BACKGROUND ART
Fungi, particularly filamentous fungi have a wide variety of enzymes for the production of secreted, the culture supernatant is used as the enzyme preparation.However, a fungal culture supernatant of the enzyme preparation in the case of using as, fungal enzyme undesirable from, for example decomposition of the objective substance into an enzyme that has become a problem.Undesirable enzyme from the culture supernatant is removed, and the like because of the need for purification or separation processes, is derived from fungal enzyme preparation, high cost, amount of production is difficult to stably supply 2 is not constant and is one major problem.In addition, it is not desirable as a method of reducing the amount of enzyme, the gene encoding the enzyme to break the lines may also be considered a method of using, in this method of fungal growth possibility is significantly reduced.Therefore, a significant proliferation of fungi without damaging, undesirable enzyme reducing the amount of the culture supernatant has been a need for a method to obtain.
On the other hand, the GPI (glycosylphosphatidylinositol), by post-translational modification of proteins are added to the end C and a lipid, a protein is immobilized on the surface of certain (anchor) cell having a function.Up to this point, the GPI, a cell surface protein in yeast can be used for the display are shown (in Patent Document 1), the enzyme secretion of endogenous GPI can be applied, it is not considered at all.
  • 出願人(英語)
  • ※2012年7月以前掲載分については米国以外のすべての指定国
  • KAGOSHIMA UNIVERSITY
  • KAKUI, CO., LTD.
  • 発明者(英語)
  • TAMAKI HISANORI
  • FUTAGAMI TAIKI
  • IWAMOTO MASATAKA
国際特許分類(IPC)
指定国 National States: AE AG AL AM AO AT AU AZ BA BB BG BH BN BR BW BY BZ CA CH CL CN CO CR CU CZ DE DJ DK DM DO DZ EC EE EG ES FI GB GD GE GH GM GT HN HR HU ID IL IN IR IS JO JP KE KG KH KN KP KR KW KZ LA LC LK LR LS LU LY MA MD ME MG MK MN MW MX MY MZ NA NG NI NO NZ OM PA PE PG PH PL PT QA RO RS RU RW SA SC SD SE SG SK SL SM ST SV SY TH TJ TM TN TR TT TZ UA UG US UZ VC VN ZA ZM ZW
ARIPO: BW GH GM KE LR LS MW MZ NA RW SD SL SZ TZ UG ZM ZW
EAPO: AM AZ BY KG KZ RU TJ TM
EPO: AL AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HR HU IE IS IT LT LU LV MC MK MT NL NO PL PT RO RS SE SI SK SM TR
OAPI: BF BJ CF CG CI CM GA GN GQ GW KM ML MR NE SN ST TD TG
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