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DATA CREATION METHOD AND DATA USE METHOD NEW

外国特許コード F180009507
整理番号 (S2017-0202-N0)
掲載日 2018年11月2日
出願国 世界知的所有権機関(WIPO)
国際出願番号 2017JP047414
国際公開番号 WO 2018117273
国際出願日 平成29年12月22日(2017.12.22)
国際公開日 平成30年6月28日(2018.6.28)
優先権データ
  • 特願2016-249896 (2016.12.22) JP
発明の名称 (英語) DATA CREATION METHOD AND DATA USE METHOD NEW
発明の概要(英語) Provided is a data creation method which includes: an autofluorescence data generation step for generating autofluorescence data that includes intensity data and/or spectrum data of autofluorescence light, by disposing the focal point of light of a prescribed wavelength at a single set of coordinates on a prescribed focal plane, irradiating a sample positioned at the coordinates with excitation light comprising light, and thereby acquiring autofluorescence light originating from the sample; a reflected light data generation step for generating intensity data of reflected light by acquiring reflected light dispersed by the sample by irradiating the single set of coordinates on the prescribed focal plane with irradiation light; and a correspondence data generation step for creating correspondence data in which the autofluorescence data at the single set of coordinates on the prescribed focal plane and the reflected light data are associated.
従来技術、競合技術の概要(英語) BACKGROUND ART
In the prior art, is the identification of the microbial species, Koch's law as organized, to isolate and culture the microorganism of the sample generally.On the other hand, the culture of microorganisms without isolation as a technique to identify microbial species, the next sequencer is based on the identification of microbial species genomic analysis meta type identification method has been proposed (for example, see Patent Document 1).According to Patent Document 1, the base sequence determined by genomic analysis meta and, comparing the nucleotide sequence of the known microorganisms by, microorganisms present in the species of microorganisms in a sample can be identified.
As a technique to identify microbial species in addition to this, the auto-fluorescence emitted by the colonies of the microbial species identification by detecting a known method (for example, Patent Document 2, reference 3).Patent Document 2, according to 3, non-invasively with respect to microorganisms microbial species can be detected.Further, a fluorescent stained cells by radiating excitation light obtained by imaging the fluorescence emitted from the cells in the captured image, based on the excitation lights of different wavelength band of the plurality of captured images using a known technique is to detect the cells (for example, see Patent Document 4).
In recent years, in order to explore the behavior of the microorganism, the confocal microscope of the microorganisms in the three-dimensional space position and recognizes the movement in the space being studied (for example, Non-Patent Document 1, reference 2).These studies, for example produced by a microbial population of microorganisms of the extracellular matrix component (biofilms) a complex three-dimensional formation of the over the observation course possible and great achievements have been in, the identification of microorganisms based on the fluorescence method, the idea is applied in the evaluation has not been reported.In addition, in the above-mentioned patent document 1-4, the spatial position in three dimensional space and recognizes the movement in the space such as, in addition to the identification of microbial species, non-invasive microorganisms and obtains the position information has not been taken into consideration.Further, in one set of coordinates on the space 1, and the fluorescence emitted from the sample is recorded in association with both of the reflected light is the idea, the above-mentioned prior art document does not describe or suggest.
  • 出願人(英語)
  • ※2012年7月以前掲載分については米国以外のすべての指定国
  • UNIVERSITY OF TSUKUBA
  • 発明者(英語)
  • Nomura Nomihiko
国際特許分類(IPC)
指定国 National States: AE AG AL AM AO AT AU AZ BA BB BG BH BN BR BW BY BZ CA CH CL CN CO CR CU CZ DE DJ DK DM DO DZ EC EE EG ES FI GB GD GE GH GM GT HN HR HU ID IL IN IR IS JO JP KE KG KH KN KP KR KW KZ LA LC LK LR LS LU LY MA MD ME MG MK MN MW MX MY MZ NA NG NI NO NZ OM PA PE PG PH PL PT QA RO RS RU RW SA SC SD SE SG SK SL SM ST SV SY TH TJ TM TN TR TT TZ UA UG US UZ VC VN ZA ZM ZW
ARIPO: BW GH GM KE LR LS MW MZ NA RW SD SL SZ TZ UG ZM ZW
EAPO: AM AZ BY KG KZ RU TJ TM
EPO: AL AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HR HU IE IS IT LT LU LV MC MK MT NL NO PL PT RO RS SE SI SK SM TR
OAPI: BF BJ CF CG CI CM GA GN GQ GW KM ML MR NE SN ST TD TG
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