TOP > 外国特許検索 > CELL CULTURE VESSEL, SAMPLE CELL FOR OBSERVATION, AND CELL CULTURE METHOD

CELL CULTURE VESSEL, SAMPLE CELL FOR OBSERVATION, AND CELL CULTURE METHOD NEW

外国特許コード F180009509
整理番号 (S2017-0041-N0)
掲載日 2018年11月2日
出願国 世界知的所有権機関(WIPO)
国際出願番号 2017JP043675
国際公開番号 WO 2018150689
国際出願日 平成29年12月5日(2017.12.5)
国際公開日 平成30年8月23日(2018.8.23)
優先権データ
  • 特願2017-026036 (2017.2.15) JP
発明の名称 (英語) CELL CULTURE VESSEL, SAMPLE CELL FOR OBSERVATION, AND CELL CULTURE METHOD NEW
発明の概要(英語) The present invention provides a cell culture vessel/sample cell for observation with which it is possible to three-dimensionally culture cells or cell tissues and understand the three-dimensional structure of the cultured cells or cell tissues easily by multifaceted observation. The cell culture vessel/sample cell for observation of the present invention is a cell culture vessel/sample cell for observation for housing a culture gel in which cells or cell tissues are embedded, the cell culture vessel/sample cell for observation characterized by: having a frame part, at least one window part surrounded by the frame part, and at least one convex part protruding from the frame part to the inside of the cell culture vessel/sample cell for observation; and having as feature points that at least one window part is translucent, has nutrient component permeability, and is provided so as to permit multifaceted observation of the cells or cell tissues, and the convex part is placed at a location that can be observed from the window part together with the cells or cell tissues.
従来技術、競合技術の概要(英語) BACKGROUND ART
The three-dimensional tissue culture cells in the gel by culturing blood vessel tissue, bronchial tissues are studies have been conducted to form a film (for example, see Patent Document 1-3).In these studies, usually, in the culture dish or well in the gel layer is formed thick, a cell or tissue culture gel layer embedding the cultured cells and tissue.As observation techniques of tissue cultured cells, cultured in an upright microscope or an inverted microscope gel layer from the underside or top side of the methods for observing the tissue is taken.In Patent Document 3, a confocal laser microscope to observe the cells stained with the dye and.A confocal laser microscope, the focused laser beam by the objective lens are connected to the cells, the fluorescent labeled cells in the focus and the auto-fluorescence of the substance can be detected.For this reason, the focal plane (xy plane) of a plurality of two-dimensional image of the z direction by overlapping the three-dimensional image of the cells can be created.
  • 出願人(英語)
  • ※2012年7月以前掲載分については米国以外のすべての指定国
  • OSAKA PREFECTURE UNIVERSITY PUBLIC CORPORATION
  • NATIONAL UNIVERSITY CORPORATION KYUSHU INSTITUTE OF TECHNOLOGY
  • 発明者(英語)
  • Masaya Hagiwara
  • Tomohiro Kawahara
国際特許分類(IPC)
指定国 National States: AE AG AL AM AO AT AU AZ BA BB BG BH BN BR BW BY BZ CA CH CL CN CO CR CU CZ DE DJ DK DM DO DZ EC EE EG ES FI GB GD GE GH GM GT HN HR HU ID IL IN IR IS JO JP KE KG KH KN KP KR KW KZ LA LC LK LR LS LU LY MA MD ME MG MK MN MW MX MY MZ NA NG NI NO NZ OM PA PE PG PH PL PT QA RO RS RU RW SA SC SD SE SG SK SL SM ST SV SY TH TJ TM TN TR TT TZ UA UG US UZ VC VN ZA ZM ZW
ARIPO: BW GH GM KE LR LS MW MZ NA RW SD SL SZ TZ UG ZM ZW
EAPO: AM AZ BY KG KZ RU TJ TM
EPO: AL AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HR HU IE IS IT LT LU LV MC MK MT NL NO PL PT RO RS SE SI SK SM TR
OAPI: BF BJ CF CG CI CM GA GN GQ GW KM ML MR NE SN ST TD TG

PAGE TOP

close
close
close
close
close
close