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METHOD AND KIT FOR CRYOPRESERVING XENOPUS LAEVIS EGG EXTRACT, CONCENTRATED XENOPUS LAEVIS EGG EXTRACT, AND METHOD FOR ANALYZING CELL CYCLE NEW

外国特許コード F180009513
整理番号 (S2017-0321-N0)
掲載日 2018年11月2日
出願国 世界知的所有権機関(WIPO)
国際出願番号 2018JP002546
国際公開番号 WO 2018139601
国際出願日 平成30年1月26日(2018.1.26)
国際公開日 平成30年8月2日(2018.8.2)
優先権データ
  • 特願2017-014441 (2017.1.30) JP
発明の名称 (英語) METHOD AND KIT FOR CRYOPRESERVING XENOPUS LAEVIS EGG EXTRACT, CONCENTRATED XENOPUS LAEVIS EGG EXTRACT, AND METHOD FOR ANALYZING CELL CYCLE NEW
発明の概要(英語) A method for cryopreserving a Xenopus laevis egg extract, characterized by comprising a partition step for partitioning the Xenopus laevis egg extract into water and solid matters and a freezing step for separately freezing the water and the solid matters.
従来技術、競合技術の概要(英語) BACKGROUND ART
Xenopus egg extracts is, the highly tuned to the cell-free cell cycle (cell-free extracts) the only experimental system can be realized.In this experimental system, using an antibody specific for a particular factor may be removed from the system and cell cycle progression (immune removed) by the control, as well as DNA replication and distribution control of the factors necessary for the unambiguous identification.
In addition, in cell cycle control and understanding of the mechanism, by using a yeast single powerful means and research, genes involved in these processes have been identified in turn.However, the eggs Xehopiis using extract cell-free system, as compared to yeast excellent in the following points. (1) Biochemical, physiological studies are suitable: for example, entity M phase promoting factor of the complex MPF cyclinB/Cdk1, Xenopus egg extracts used by the cell-free system, have been identified for the first time (for example, see Non-Patent Document 1.). (2) In vitro in the partial reconstruction. (3) Different yeast and multicellular animal and the frog is, closer to the human model.To this, the cell-free system using Xenopus egg extracts by, the cell cycle specific multicellular animal control mechanism has been revealed.
  • 出願人(英語)
  • ※2012年7月以前掲載分については米国以外のすべての指定国
  • INTER-UNIVERSITY RESEARCH INSTITUTE CORPORATION RESEARCH ORGANIZATION OF INFORMATION AND SYSTEMS
  • 発明者(英語)
  • Jun Takagi
国際特許分類(IPC)
指定国 National States: AE AG AL AM AO AT AU AZ BA BB BG BH BN BR BW BY BZ CA CH CL CN CO CR CU CZ DE DJ DK DM DO DZ EC EE EG ES FI GB GD GE GH GM GT HN HR HU ID IL IN IR IS JO JP KE KG KH KN KP KR KW KZ LA LC LK LR LS LU LY MA MD ME MG MK MN MW MX MY MZ NA NG NI NO NZ OM PA PE PG PH PL PT QA RO RS RU RW SA SC SD SE SG SK SL SM ST SV SY TH TJ TM TN TR TT TZ UA UG US UZ VC VN ZA ZM ZW
ARIPO: BW GH GM KE LR LS MW MZ NA RW SD SL SZ TZ UG ZM ZW
EAPO: AM AZ BY KG KZ RU TJ TM
EPO: AL AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HR HU IE IS IT LT LU LV MC MK MT NL NO PL PT RO RS SE SI SK SM TR
OAPI: BF BJ CF CG CI CM GA GN GQ GW KM ML MR NE SN ST TD TG

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