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MUSCLE DIFFERENTIATION PROMOTER, MUSCLE DIFFERENTIATION PROMOTION METHOD, MUSCLE-DIFFERENTIATION-PROMOTING OLIGO DNA, ENHANCER, AND OLIGO DNA commons

Foreign code F180009527
File No. (L16020,L17008,S2017-0356-N0)
Posted date Nov 2, 2018
Country WIPO
International application number 2018JP005305
International publication number WO 2018151225
Date of international filing Feb 15, 2018
Date of international publication Aug 23, 2018
Priority data
  • P2017-026547 (Feb 16, 2017) JP
  • P2017-150320 (Aug 3, 2017) JP
Title MUSCLE DIFFERENTIATION PROMOTER, MUSCLE DIFFERENTIATION PROMOTION METHOD, MUSCLE-DIFFERENTIATION-PROMOTING OLIGO DNA, ENHANCER, AND OLIGO DNA commons
Abstract The muscle differentiation promoter according to the present invention includes oligo DNA having activity to promote muscle differentiation by being applied to cells or to an individual. The oligo DNA preferably includes a core base sequence represented by 5'TTAGGG3' or 5'TGAGGG3' and has a base sequence AGA or a base sequence AAG on the 5' end side. Berberine or an analogous compound thereof or a salt thereof may be included in addition to the oligo DNA.
Outline of related art and contending technology BACKGROUND ART
Super senior society, age and skeletal muscle atrophy main features including the rapid increase in the syndrome and the sarcopenia, are a significant problem as the cause of the bedridden.The recent studies have shown that, of the skeletal muscle atrophy independent of prognosis of heart failure such as a risk factor, the enhancement of motor function and/or treatment of various diseases is also important for the prevention of being revealed.Many elderly people are standing lives in order to realize the society health longevity, strength and/or muscle mass over the life thereof is essential to maintain.Age is in the prevention of muscle atrophy, muscle beneficial molecule via a meal such as ingestion, and long term efforts routinely considered to be effective, safe and inexpensive is demanded of a functional molecule.
The skeletal muscle, skeletal muscle stem cells are referred to as satellite cells of the played back by the growth and differentiation, as tissue homeostasis is maintained.Aging progresses, in the muscle tissue of the reduction in the number of satellite cells, in addition, the regeneration ability of the individual satellite cells also decreases.To suppress the aging of the satellite cells, activates the playback capability to identify and search for the functional molecules, age-related muscle atrophy of a new strategy is expected to lead to the proposal.
For example, to mouse skeletal muscle intrafetally extracted from the satellite cells obtained using the myoblasts, skeletal muscle differentiation screening system is a molecule which has been established, this screening by the system, included in one of the polyphenols turmeric curcumin to promote differentiation of muscle has been found (Non-Patent Document 1).
However, naturally curcumin, such as a raw material has been cultivated and turmeric, organic solvent extraction method, an alcohol such as extraction, separation and extraction is performed, and even the current produced is carried out, with respect to an increase in the future worldwide demand for high purity can be produced at a low cost stable carcumin whether or not there is a concern.Patent Document 1 due to a defect in the method of DNA-DNA repair and cancer of the present invention for the purpose of suppression.
Further, from the viewpoint of anti-aging benefits, telomere homologous oligonucleotides (SEQ ID NO:17, 18) by exposure of the cells, a technology for increasing the average telomere length (Patent Document 1) has been disclosed.
Scope of claims (In Japanese)請求の範囲
[請求項1]
 細胞又は個体に対して適用することにより筋分化を促進する活性を有するオリゴDNAを含む筋分化促進剤。
[請求項2]
 ベルベリンもしくはその類縁体化合物又はその塩をさらに含む請求項1に記載の筋分化促進剤。
[請求項3]
 前記オリゴDNAは、5’TTAGGG3’又は5’TGAGGG3’で表されるコア塩基配列が2以上連結している、請求項1又は2記載の筋分化促進剤。
[請求項4]
 前記オリゴDNAは、5’TTAGGG3’で表されるコア塩基配列に5’TTAGGG3’又は5’TGAGGG3’で表されるコア塩基配列を連結して含む請求項3記載の筋分化促進剤。
[請求項5]
 前記オリゴDNAは、塩基配列5’TTAGGGTGAGGG3’を含む請求項4記載の筋分化促進剤。
[請求項6]
 前記オリゴDNAは、5’TTAGGG3’ 又は5’TGAGGG3’で表されるコア塩基配列を含み、5’末端側に塩基配列AGA又は塩基配列AAGを有する請求項1記載の筋分化促進剤。
[請求項7]
 哺乳類又は鳥類の細胞又は個体に対して適用するための請求項1から6のいずれか1項に記載の筋分化促進剤。
[請求項8]
 前記細胞はマウス筋芽細胞又はニワトリ筋芽細胞又はヒト横紋筋肉腫細胞である請求項7記載の筋分化促進剤。
[請求項9]
 前記個体はマウス、ニワトリ又はヒトである請求項7記載の筋分化促進剤。
[請求項10]
 前記オリゴDNAは5’TTAGGG3’で表されるコア塩基配列を含む請求項2記載の筋分化促進剤。
[請求項11]
 5’末端側に塩基配列AGA又は塩基配列AAGを有した請求項10記載の筋分化促進剤。
[請求項12]
 前記オリゴDNAと前記ベルベリンもしくはその類縁体化合物又はその塩のモル比は1:10~10:1である、請求項9記載の筋分化促進剤。
[請求項13]
 請求項1から12のいずれかの1項に記載の筋分化促進剤を使用する筋分化促進方法。
[請求項14]
 5’TTAGGG3’又は5’TGAGGG3’で表されるコア塩基配列を含み、5’末端側に塩基配列AGA又は塩基配列AAGを有するた筋分化を促進する活性を有するオリゴDNA。
[請求項15]
 5’TTAGGG3’又は5’TGAGGG3’で表されるコア塩基配列が2以上連結している請求項14記載のオリゴDNA。
[請求項16]
 5’TTAGGG3’で表されるコア塩基配列に5’TTAGGG3’又は5’TGAGGG3’で表されるコア塩基配列を連結して含む請求項15記載のオリゴDNA。
[請求項17]
 塩基配列5’TTAGGGTGAGGG3’を含む請求項16記載のオリゴDNA。
[請求項18]
 細胞又は個体に対して適用することにより筋分化を促進する活性を有し、塩基長が6から25のいずれかであるオリゴDNA。
[請求項19]
 前記塩基長が9から18のいずれかである請求項18記載のオリゴDNA。
[請求項20]
 前記塩基長が18である請求項18記載のオリゴDNA。
[請求項21]
 5’TTAGGG3’又は5’TGAGGG3’で表されるコア塩基配列を含み、5’末端側に塩基配列AGA又は塩基配列AAGを有した請求項18記載のオリゴDNA。
[請求項22]
 5’TTAGGG3’又は5’TGAGGG3’で表されるコア塩基配列が2以上連結したことを特徴とする請求項19記載のオリゴDNA。
[請求項23]
 オリゴDNAによる筋分化促進を増強するための増強剤であって、ベルベリンもしくはその類縁化合物又はその塩を含む増強剤。
[請求項24]
 前記オリゴDNAは5’TTAGGG3’で表されるコア塩基配列を含む請求項23記載の増強剤。
  • Applicant
  • ※All designated countries except for US in the data before July 2012
  • SHINSHU UNIVERSITY
  • Inventor
  • TAKAYA, Tomohide
  • SHIMOSATO, Takeshi
  • UMEZAWA, Koji
IPC(International Patent Classification)
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