NOVEL TISSUE REGENERATION MATERIAL AND METHOD FOR PRODUCING SAME
|Posted date||Nov 2, 2018|
|International application number||2018JP000577|
|International publication number||WO 2018131673|
|Date of international filing||Jan 12, 2018|
|Date of international publication||Jul 19, 2018|
|Title||NOVEL TISSUE REGENERATION MATERIAL AND METHOD FOR PRODUCING SAME|
|Abstract||The present invention pertains to a novel tissue regeneration material and a method for producing the same. The method for producing the tissue regeneration material comprises the following steps: (1) mixing a solution or dispersion of collagen with a neutral culture medium containing stem cells; (2) centrifuging the mixture obtained in (1) and thus accumulating the stem cells and collagen; and (3) culturing the accumulated matter consisting of the stem cells and collagen.|
|Outline of related art and contending technology||
Cartilage or ligament modified by the aging or sports, an increase in the damage is increasing steadily.Joint in the tendon, ligament, cartilage of a joint that stabilizes an important role on the other hand, once the self-healing and damage have the characteristic.These tissue is damaged, the body is treated by implanting the same are often performed, repair tissue mechanical properties is poor compared to normal tissue is a problem. 30% Or more of the population suffering from osteoarthritis or the like, to maintain the QOL, in order to improve tissue repair and to improve the quality level has been demanded.
As the most promising material for the repair of the iPS cells of mesenchymal stem cells such as stem cells and stem cell-derived tissue repair the basic material.Stem cell growth and differentiation ability (the ability to increase the self) (the ability to other cells) and cells with together with a generic, all in theory can be to create a living tissue, stem cells and tissue repair material based approach is very promising.Mesenchymal stem cells from a plurality of stem cells is present in a living body which is a kind of the somatic stem cells, can be easily collected from an individual, for the risk of rejection, of regenerative medicine has attracted attention as a realistic trump.
For example, tendon, ligament, cartilage repair approach solves the problem, the knee synovial taken from the mesenchymal stem cells (MSCs) such as extracellular matrix of collagen (extracellular matrix: ECM) produced by the self-generating and self-generated tissue (stem cell-based self-assembled tissue stem cells: scSAT) has been developed, basic research has been performed (non-patent document 1).ScSAT is, the tissue in the art, an engineered, construct (tissue engineered construct: TEC) also known as.ScSAT is, animal-derived collagen or artificial scaffold compound is not required, when embedded in a living body such as a rejection feature such as less likely.Cartilage repair scSAT to this effect have been reported (Non-Patent Document 2).However, it is self-generated cells and scSAT extracellular matrix organization and is, in the field of transplantation surgery and the like easy to tear a thin handling difficulties in addition to the above mentioned problem, the dynamics of the normal cartilage tissue repair is low in comparison with the, particularly the surface normal does not increase as the concentration of collagen is the cartilage has been a problem (non-patent document 3).
However, including the techniques described above, the mesenchymal stem cell-derived tissue regeneration material 3 of one of the following problem.A) differentiating stem cells from the culture environment such as the constraints of the potential of the device main body is not the maximum.B) low mechanical strength of the tissue regeneration, healing ability is low and the surrounding tissue.C) the tissue can be prepared for a small volume of recycled material cannot be used to treat a wide range.
Tissue regeneration material in order to improve the mechanical strength, some cells and creating a composite of the scaffold can be considered, in general, a scaffold for the creation of the complex of cells, the cells are allowed to invade into the scaffold has been difficult.For example, and the load of the negative pressure by the back-flow of the culture solution, the cells are allowed to invade into the scaffold are performed studies, these methods are less effective, as biological tissue containing cells and uniform to a certain level such as high density state cannot be created.
Yokoyama et al., in the collagen collagen culturing the cells in a culture method, low molecular weight collagen matrix containing a large amount of stem cells to be successful (non-patent document 4).This growth method, the way existing cell culture such as gelatin (nitta corporation) nitta corporation, a commercially available product may also be present.This method is well within the scaffold to enter the cell from the viewpoint, according to the present invention is to solve the above problem, in this method 106 cells/cm2 or more large amount of cells are required, the invention may be generated in a small amount of material, low molecular weight having a low degree of fibrosis-fiber in most cases.Thus a small amount of cells, in vivo than a high degree of fibrosis in an environment close to a large amount of collagen fibers contained in the cell at a high density is impossible.
|Scope of claims||
工程（1）の幹細胞が、1．0×10 5細胞／cm 2～6．0×10 5細胞／cm 2の細胞密度まで培養した幹細胞である、請求項1または2に記載の方法。
|IPC(International Patent Classification)||
National States: AE AG AL AM AO AT AU AZ BA BB BG BH BN BR BW BY BZ CA CH CL CN CO CR CU CZ DE DJ DK DM DO DZ EC EE EG ES FI GB GD GE GH GM GT HN HR HU ID IL IN IR IS JO JP KE KG KH KN KP KR KW KZ LA LC LK LR LS LU LY MA MD ME MG MK MN MW MX MY MZ NA NG NI NO NZ OM PA PE PG PH PL PT QA RO RS RU RW SA SC SD SE SG SK SL SM ST SV SY TH TJ TM TN TR TT TZ UA UG US UZ VC VN ZA ZM ZW
ARIPO: BW GH GM KE LR LS MW MZ NA RW SD SL SZ TZ UG ZM ZW
EAPO: AM AZ BY KG KZ RU TJ TM
EPO: AL AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HR HU IE IS IT LT LU LV MC MK MT NL NO PL PT RO RS SE SI SK SM TR
OAPI: BF BJ CF CG CI CM GA GN GQ GW KM ML MR NE SN ST TD TG
Contact Information for " NOVEL TISSUE REGENERATION MATERIAL AND METHOD FOR PRODUCING SAME "
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