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CELL SORTING METHOD NEW

外国特許コード F180009566
整理番号 (S2017-0495-N0)
掲載日 2018年11月5日
出願国 世界知的所有権機関(WIPO)
国際出願番号 2018JP008615
国際公開番号 WO 2018164133
国際出願日 平成30年3月6日(2018.3.6)
国際公開日 平成30年9月13日(2018.9.13)
優先権データ
  • 特願2017-043744 (2017.3.8) JP
発明の名称 (英語) CELL SORTING METHOD NEW
発明の概要(英語) Provided is a novel simplified and easy cell separation method. Also provided are: a cell sorting method, comprising (a) performing a treatment for increasing the difference in cell membrane fluidity between a target cell and a non-target cell and (b) sorting the target cell utilizing the difference in cell membrane fluidity; a kit for cell sorting; a culture medium for use in cell sorting; a reagent for performing a treatment for increasing the difference in cell membrane fluidity between a target cell and a non-target cell; and a method for quantifying the adhesion of a cell to a substrate.
従来技術、競合技術の概要(英語) BACKGROUND ART
Takebe et al. develop liver initial occurrence of a phenomenon relating to the processes 3 one of the cells (formed in) are identified, they may be mixed in a liver in vitro (organ type) to create the original group of successful (non-patent document 1:Nature 2013, Patent Document 1:WO2013/047639 A1: a method of producing the tissues and organs) of the original group is liver. for the expression of a liver function after implantation, for patients with organ dysfunction have been spotlighted as a therapy epoch. in addition, the method of the same intestine, kidney, pancreas, brain, lung, cancer, such as the heart, can be extended to a variety of tissues (JP-2:WO2015/012158 A1, JP-3:WO2015/129822 A1) from, the original group of the base to construct the principle of the organ, is expected to be applied to various fields and where., is applied to the actual organ regeneration for medical, the safety and the homogeneity of the organ to establish a method to induce the original group is necessary. however, as a point of major problem with the present system, cells in the raw material 'safety' and the 'uniformity' of the original group is a problem such as a human organ. iPS cells from stem cells by adjusting the material and a plurality of cells, is capable of inducing, iPS undifferentiated cells, such as midway in the undifferentiated progenitor cells of the non-target cells may be mixed therein and, safety and uniformity problems have been left for example., this remaining undifferentiated cells and to cancer, the risk to grow although it has been shown that, in the experiment by a simple and easy user level is an undifferentiated cell separation method is not established (non-patent document 2: the Cunningham et al., Nat.Biothechnol. 2012)., and endodermal cells in the midway of the non-target cells to differentiate into the gut and intestinal tissue may be formed in the remaining, stromal cells in the same manner as the remaining excess fibrotic tissue can be formed although the risk of such as pointed out, such a tissue progenitor cells are selectively removed from the cell source method does not exist in the actual laboratory level., a number of donor cells of the organ using the original group of researchers that function depends on the stoichiometry multilaterally analyze the experience-and the method, on the industrial applications has been a crucial barrier and reproduced as a cell sorting. medical research, drug discovery is widely used in the field of research or Fluorescence activated cell sorting (FACS), is Magnetic-activated cell sorting (MACS) (Non-Patent Document 3: corresponding to surface antigens Maecker et al., Nature Immunology 2010). the present method is applied to a magnetic material and a fluorescent antibody reaction, fluorescence and magnetic force can be isolated selectively only emits cells. however, the selection of fluorescent antibodies to the identification of surface antigens, and the enormous amount of time to purchase further FACS the cost also., the actual field of regenerative medicine in the case where the assumed to be used, solid organ such as a large amount greater than required for the treatment of (1010-) the accuracy of the cell sorting is required, the sorting takes a long time because of the damage to the cells with difficult to use in a further., and the remaining markers is also an issue of great risk assessment for the remaining unlabeled and where., low cost, the development of rapid cell selection methods have been expected.
  • 出願人(英語)
  • ※2012年7月以前掲載分については米国以外のすべての指定国
  • PUBLIC UNIVERSITY CORPORATION YOKOHAMA CITY UNIVERSITY
  • 発明者(英語)
  • Takanori Takebe
  • Hideki Taniguchi
国際特許分類(IPC)
指定国 National States: AE AG AL AM AO AT AU AZ BA BB BG BH BN BR BW BY BZ CA CH CL CN CO CR CU CZ DE DJ DK DM DO DZ EC EE EG ES FI GB GD GE GH GM GT HN HR HU ID IL IN IR IS JO JP KE KG KH KN KP KR KW KZ LA LC LK LR LS LU LY MA MD ME MG MK MN MW MX MY MZ NA NG NI NO NZ OM PA PE PG PH PL PT QA RO RS RU RW SA SC SD SE SG SK SL SM ST SV SY TH TJ TM TN TR TT TZ UA UG US UZ VC VN ZA ZM ZW
ARIPO: BW GH GM KE LR LS MW MZ NA RW SD SL SZ TZ UG ZM ZW
EAPO: AM AZ BY KG KZ RU TJ TM
EPO: AL AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HR HU IE IS IT LT LU LV MC MK MT NL NO PL PT RO RS SE SI SK SM TR
OAPI: BF BJ CF CG CI CM GA GN GQ GW KM ML MR NE SN ST TD TG
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