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MICROSCOPIC SUBSTANCE ENCAPSULATION METHOD, MICROSCOPIC SUBSTANCE DETECTION METHOD, AND DEVICE FOR DETECTING MICROSCOPIC SUBSTANCE NEW

外国特許コード F180009585
整理番号 AF19-15WO
掲載日 2018年11月7日
出願国 世界知的所有権機関(WIPO)
国際出願番号 2018JP012678
国際公開番号 WO 2018181443
国際出願日 平成30年3月28日(2018.3.28)
国際公開日 平成30年10月4日(2018.10.4)
優先権データ
  • 特願2017-064789 (2017.3.29) JP
発明の名称 (英語) MICROSCOPIC SUBSTANCE ENCAPSULATION METHOD, MICROSCOPIC SUBSTANCE DETECTION METHOD, AND DEVICE FOR DETECTING MICROSCOPIC SUBSTANCE NEW
発明の概要(英語) The present invention provides, as a technology for encapsulating, in a tiny volume of liquid droplet, a to-be-detected substance such as nucleic acid, protein, virus, or cell by means of a simple operation so as to enable highly sensitive detection, a method for encapsulating a microscopic substance in at least some of a plurality of recesses formed in the surface of a substrate so as to be spaced away from each other, the method comprising (1) a first step for disposing an insertion body above the recess-formed surface of the substrate, positioning the relative positions of said insertion body and the substrate by a supporting part provided to the insertion body in such a manner as to have the undersurface of the insertion body and the recess-formed surface of the substrate face each other, thereby providing a solution introduction space between the undersurface of the insertion body and the recess-formed surface of the substrate, and providing a solution discharge space that is connected with the solution introduction space and is provided above the undersurface of the insertion body and between the substrate and the insertion body so as to be positioned within the substrate and/or within the insertion body.
従来技術、競合技術の概要(英語) BACKGROUND ART
For the diagnosis of a disease or infection such as, nucleic acid, protein, viruses and cell markers such as a rapid, simple, and high-sensitivity detection is demanded in the art.For example, 100 million the volume of the tumor contained in the 1mm3 cancer cells (each cell from each 100 molecule) is a marker protein is secreted into the blood of the case 5L, the blood concentration of the marker protein is about 30aM.Such a very low concentration of a substance is demanded a technique which can be detected.
As such techniques, nucleic acid, protein, viruses and cells such as the detection object substance is sealed in a minimum volume of liquid droplets, using a labeled antibody is detected by immunological method 'of an enzyme molecule' is present.According to the enzyme molecule, one molecule of the substance to be detected can be detected with the sensitivity of the unit.
Patent Document 1 is, as a technique which is applicable to single-molecule enzyme assay, '1 can accommodate only one of the plurality of beads of the housing part, the hydrophobic upper surface are spaced from each other by the side wall is formed between the lower layer, the accommodating portion of the lower portion is formed in the surface facing the space between the upper layer, the hydrophilic solvent including beads is introduced into the introducing beads, after the step of introducing the beads into the space above the hydrophobic solvent and hydrophobic solvent introducing step of introducing encapsulating beads characterized in that it contains a method' is disclosed.
The technique disclosed in Patent Document 1, 'the plurality of accommodation parts hydrophobic top surface are spaced from each other by the side wall is formed between the lower layer, the accommodating portion is formed in the lower layer described above with respect to the surface being opposed to each other with a space provided between the upper portion of the array and' is used and, the upper and the lower space has a structure opposite to the flow cell in the array is used.According to this technique, 'a large number of beads in an array can be efficiently enclosed, a low concentration of target molecules can be detected with high sensitivity' therein.
  • 出願人(英語)
  • ※2012年7月以前掲載分については米国以外のすべての指定国
  • JAPAN SCIENCE AND TECHNOLOGY AGENCY
  • 発明者(英語)
  • Hiroyuki Noji
  • Kazuhito Tabata
国際特許分類(IPC)
指定国 National States: AE AG AL AM AO AT AU AZ BA BB BG BH BN BR BW BY BZ CA CH CL CN CO CR CU CZ DE DJ DK DM DO DZ EC EE EG ES FI GB GD GE GH GM GT HN HR HU ID IL IN IR IS JO JP KE KG KH KN KP KR KW KZ LA LC LK LR LS LU LY MA MD ME MG MK MN MW MX MY MZ NA NG NI NO NZ OM PA PE PG PH PL PT QA RO RS RU RW SA SC SD SE SG SK SL SM ST SV SY TH TJ TM TN TR TT TZ UA UG US UZ VC VN ZA ZM ZW
ARIPO: BW GH GM KE LR LS MW MZ NA RW SD SL SZ TZ UG ZM ZW
EAPO: AM AZ BY KG KZ RU TJ TM
EPO: AL AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HR HU IE IS IT LT LU LV MC MK MT NL NO PL PT RO RS SE SI SK SM TR
OAPI: BF BJ CF CG CI CM GA GN GQ GW KM ML MR NE SN ST TD TG
参考情報 (研究プロジェクト等) CREST Creation of Nanosystems with Novel Functions through Process Integration AREA
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