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METHOD FOR INDUCING MYOCARDIAL DIFFERENTIATION OF PLURIPOTENT STEM CELLS USING LOW-MOLECULAR COMPOUND UPDATE

外国特許コード F180009642
整理番号 4592
掲載日 2018年11月21日
出願国 世界知的所有権機関(WIPO)
国際出願番号 2015JP065643
国際公開番号 WO 2015182765
国際出願日 平成27年5月29日(2015.5.29)
国際公開日 平成27年12月3日(2015.12.3)
優先権データ
  • 特願2014-113325 (2014.5.30) JP
発明の名称 (英語) METHOD FOR INDUCING MYOCARDIAL DIFFERENTIATION OF PLURIPOTENT STEM CELLS USING LOW-MOLECULAR COMPOUND UPDATE
発明の概要(英語) The present invention is a method for inducing the myocardial differentiation of pluripotent stem cells wherein the method includes (1) a step for culturing pluripotent stem cells in a medium containing a WNT signal activator and PKC activator, and (2) a step for culturing the cells obtained in step (1) in medium containing a WNT signal inhibitor, Src inhibitor, and EGF receptor inhibitor.
従来技術、競合技術の概要(英語) BACKGROUND ART
1 Cause of death in the world and at heart disease, heart transplantation in patients with severe heart failure only treatment but, that suffers from shortage of donors. An alternative to heart transplantation as a therapy, such as ES cells or iPS cells derived from pluripotent stem cells and cardiac muscle cell transplantation is a promising, quickly implementation is desired. However, the pluripotent stem cell-derived myocardial cells is for application to regenerative medicine, a major problem that the production cost and safety must be solved.
Is implanted in a human cardiac muscle cells, at least 10 billion (10 9 of the n-th power) of mass cell requirement for the considered. However, the current method to induce the differentiation of pluripotent stem cells of the myocardium is -, growth factor or cytokine, such as serum albumin protein due to the need for a large amount, an extremely high cost. Specifically, 9 of the n-th power of 10 required for producing a cardiomyocyte culture medium only (10 liter), the estimated cost of 1 million yen or more becomes. In fact, part of the current commercially available human pluripotent stem cell derived from cardiac muscle cells are an extremely small capacity and expensive (10 6 to the square of the scale of several hundred thousand yen) and, 10 needed to implant the cells of the n-th power of 9 to several hundreds of million yen and calculation. Therefore, the cost of the myocardial differentiation induction medium is reduced, the heart is extremely important in the realization of regenerative medicine has been a problem.
In addition, in the aspect of safety, the current to induce the differentiation of cytokine or protein in the culture medium becomes a problem. All these proteins can be, an animal cell or a bacterium, yeast or the like and is hence, risk of infection from its original cells (virus, mycoplasma, prions etc.) occurs. Myocardial differentiation induction is particularly, for one step is a step before transplantation, infection risk here should be reduced as much as possible.
  • 出願人(英語)
  • ※2012年7月以前掲載分については米国以外のすべての指定国
  • KYOTO UNIVERSITY
  • 発明者(英語)
  • NAKATSUJI, Norio
  • MINAMI, Itsunari
国際特許分類(IPC)
指定国 National States: AE AG AL AM AO AT AU AZ BA BB BG BH BN BR BW BY BZ CA CH CL CN CO CR CU CZ DE DK DM DO DZ EC EE EG ES FI GB GD GE GH GM GT HN HR HU ID IL IN IR IS JP KE KG KN KP KR KZ LA LC LK LR LS LU LY MA MD ME MG MK MN MW MX MY MZ NA NG NI NO NZ OM PA PE PG PH PL PT QA RO RS RU RW SA SC SD SE SG SK SL SM ST SV SY TH TJ TM TN TR TT TZ UA UG US UZ VC VN ZA ZM ZW
ARIPO: BW GH GM KE LR LS MW MZ NA RW SD SL SZ TZ UG ZM ZW
EAPO: AM AZ BY KG KZ RU TJ TM
EPO: AL AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HR HU IE IS IT LT LU LV MC MK MT NL NO PL PT RO RS SE SI SK SM TR
OAPI: BF BJ CF CG CI CM GA GN GQ GW KM ML MR NE SN ST TD TG
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