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MICROFLUID DEVICE AND THREE-DIMENSIONAL MICROCULTURE METHOD FOR CELL UPDATE

外国特許コード F180009647
整理番号 4484
掲載日 2018年11月21日
出願国 世界知的所有権機関(WIPO)
国際出願番号 2015JP055178
国際公開番号 WO 2015129673
国際出願日 平成27年2月24日(2015.2.24)
国際公開日 平成27年9月3日(2015.9.3)
優先権データ
  • 特願2014-034166 (2014.2.25) JP
発明の名称 (英語) MICROFLUID DEVICE AND THREE-DIMENSIONAL MICROCULTURE METHOD FOR CELL UPDATE
発明の概要(英語) The present invention provides a microfluid device comprising a cell culture chamber connected to at least two openings. When cells and hydrogel are introduced to the cell culture chamber and cells are cultured in a three-dimensional gel medium, biologically active substances can be fed from at least one opening to the cell culture chamber while a concentration gradient is formed inside the chamber.
従来技術、競合技術の概要(英語) BACKGROUND ART
Cells, the extracellular in vivo microenvironment under its functions in control is being performed. The extracellular microenvironment, mainly (i) growth factor, vitamin, gas molecules such as soluble factors, extracellular matrix proteins (ii), such as the hardness of the non-soluble factors, cell-cell interactions (iii), is configured from. These factors is complicated, and while the tightly controlled, cell function control is performed. That is, human ES cells and human iPS cells for the purpose of cell function in order to freely control, this extracellular microenvironment as it is necessary to freely control.
However, these control operations are performed in a micrometer scale and very small, conventional culturing of the cells, experimental method, using a culture dish or plate 2 is three-dimensional environment, cannot be reproduced. Therefore, was difficult by a conventional method, 3-dimensional cell culture, can be made to the experimental environment has been demanded.
Culturing the cell in the conventional human ES/iPS, experimental method, such as three-dimensional culture dish was performed in a simulated environment 2 (non-patent document 1, 2).
However, the original, 3 cells are placed in a three-dimensional environment, 2 expression and the function of the three-dimensional environment cannot be. Human ES/iPS cells were used in tissue engineering, the three-dimensional environment for 3 is very important.
The size and the very important factors. Cells in vivo, in micrometer scale controlled micro-environment. For example, the concentration gradient and of a soluble factor, the hardness of the extracellular matrix and the like. These factors by a conventional method was difficult to control. Of course, these factors can be comprehensively analyzed, was almost impossible.
  • 出願人(英語)
  • ※2012年7月以前掲載分については米国以外のすべての指定国
  • KYOTO UNIVERSITY
  • 発明者(英語)
  • KAMEI, Kenichiro
  • CHEN, Yong
国際特許分類(IPC)
指定国 National States: AE AG AL AM AO AT AU AZ BA BB BG BH BN BR BW BY BZ CA CH CL CN CO CR CU CZ DE DK DM DO DZ EC EE EG ES FI GB GD GE GH GM GT HN HR HU ID IL IN IR IS JP KE KG KN KP KR KZ LA LC LK LR LS LU LY MA MD ME MG MK MN MW MX MY MZ NA NG NI NO NZ OM PA PE PG PH PL PT QA RO RS RU RW SA SC SD SE SG SK SL SM ST SV SY TH TJ TM TN TR TT TZ UA UG US UZ VC VN ZA ZM ZW
ARIPO: BW GH GM KE LR LS MW MZ NA RW SD SL SZ TZ UG ZM ZW
EAPO: AM AZ BY KG KZ RU TJ TM
EPO: AL AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HR HU IE IS IT LT LU LV MC MK MT NL NO PL PT RO RS SE SI SK SM TR
OAPI: BF BJ CF CG CI CM GA GN GQ GW KM ML MR NE SN ST TD TG
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