TOP > 外国特許検索 > TISSUE SLICE SELECTION METHOD, AND EMBEDDED BLOCK PREPARING CASSETTE

TISSUE SLICE SELECTION METHOD, AND EMBEDDED BLOCK PREPARING CASSETTE NEW

外国特許コード F190009686
整理番号 (S2017-0517-N0)
掲載日 2019年1月23日
出願国 世界知的所有権機関(WIPO)
国際出願番号 2018JP017336
国際公開番号 WO 2018199335
国際出願日 平成30年4月27日(2018.4.27)
国際公開日 平成30年11月1日(2018.11.1)
優先権データ
  • 特願2017-090793 (2017.4.28) JP
発明の名称 (英語) TISSUE SLICE SELECTION METHOD, AND EMBEDDED BLOCK PREPARING CASSETTE NEW
発明の概要(英語) Provided are a tissue slice selection method and an embedded block preparing cassette with which it is possible to suppress the occurrence of errors in inspection results. This tissue slice selection method makes it possible to suppress the occurrence of errors in inspection results by comprising: an embedded block preparing step of preparing an embedded block 24 by embedding a biological tissue piece 22 and a reference material 21 in the same embedding agent 23; an embedded tissue slice preparing step of thinly cutting the embedded block 24 to prepare a sheet-like embedded tissue slice 44 in the surface of which a tissue slice 42 and a reference material slice 41a, 41b, 41c, 41d, 41e appear; and a tissue slice selecting step of selecting the tissue slice 42 to be inspected, on the basis of an optical signal from the reference material slice.
従来技術、競合技術の概要(英語) BACKGROUND ART
Cancerous tissue in a particular gene is amplified by the product of the gene is overexpressed protein is known.In the inspection of the cancer or the like, of the protein in the biological sample obtained from the over-expression or gene amplification is detected by immunohistochemical staining, the state of each specimen being inspected.
For example, such as breast cancer tumor in a cell, one of the 1 oncogene HER2/neu protein HER2/neu gene product of the gene was overexpressed in a high frequency.Therefore, using immunohistochemical staining HER2/neu protein is detected in the tumor cells, HER2/neu over-expression of the protein to determine whether or not, whether or not suffering from cancer can be inspected.
This principle is applied as the test kit, HER2/neu protein expression in tumor cells has been targeted, HER2/neu protein in the extracellular region of the anti-human monoclonal antibody recognizing HER2/neu gene product (SV2-61γ) (animal species: mouse) immunohistochemical staining using the kit is sold.
Such immunohistochemical staining kit, through the following steps are immunohistochemical staining the specimen (hereinafter, simply referred to as staining.).First, for example collected from the human body to be examined such as liquid paraffin embedded tissue piece of the embedded agent and the embedded block is prepared by preparing step, the embedding block to a desired thickness by a microtome or the like and be sectioned, the sections of the peripheral sections of the tissue surrounded by the embedded agent is embedded tissue sections of a sheet shape made of the number of manufacturing steps, and, in this way the embedded tissue sections were prepared on a glass slide and placed in the mounting step, and staining the sample can be prepared in the form.
Subsequently, on the slide glass to remove paraffin embedded tissue sections of paraffin is removed from the processing step, and, the tissue sections were activation antigen activation treatment step.
Finally, 3V/V % with a hydrogen peroxide solution and the like of the endogenous peroxidase blocking step, a specific antigen in the tissue section on the primary antibody is bound primary antibody reactive processing step, the enzyme labeled secondary antibody coupled to a primary antibody which binds to a secondary antibody reactive processing step, and, a substrate solution containing a chromogenic substrate (for example DAB: 3,3 ' - diaminobenzidine) by using the color of the stained tissue section stained with antigen-specific processing step is performed.Such a series of steps, tissue sections are stained (non-patent document 1).
In this case, staining the slide control for determining whether, in addition to the embedded tissue sections prepared in advance, the control slide stained tissue sections may be at the same time.The control slide, and a sample specimen, staining intensity of the sample and the sample can be determined whether or not a stain from.Control by means of a slide, staining was properly performed is confirmed, based on the predefined criteria is determined that the score of the intensity of the staining of tissue sections, the score from the result of the determination, whether or not the tissue in the lesion tissue sections are carried out (non-patent document 2).
  • 出願人(英語)
  • ※2012年7月以前掲載分については米国以外のすべての指定国
  • NIHON UNIVERSITY
  • NICHIREI BIOSCIENCES INC.
  • 発明者(英語)
  • MASUDA Shinobu
  • SUZUKI Ryohei
  • KITANO Yuriko
国際特許分類(IPC)
日本大学産官学連携知財センター(通称NUBIC,ニュービック)は,技術移転機関と知的財産本部の機能を兼ね備えた日本大学の産学連携の窓口です。
NUBICは,日本大学全教職員や大学院生・学部学生の豊富なアイデアや研究成果を,知的財産として戦略的に創出・保護・管理し,産業界のニーズとのマッチングを図り,企業の研究開発,新製品開発,新規事業の立上げが円滑に行われるようサポートいたします。
お気軽にご相談ください。

PAGE TOP

close
close
close
close
close
close