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METHOD FOR OBTAINING INFORMATION OF ANTIGEN-SPECIFIC ANTIBODY NEW

外国特許コード F190009687
整理番号 (S2017-0535-N0)
掲載日 2019年1月23日
出願国 世界知的所有権機関(WIPO)
国際出願番号 2018JP015671
国際公開番号 WO 2018194015
国際出願日 平成30年4月16日(2018.4.16)
国際公開日 平成30年10月25日(2018.10.25)
優先権データ
  • 特願2017-084431 (2017.4.21) JP
発明の名称 (英語) METHOD FOR OBTAINING INFORMATION OF ANTIGEN-SPECIFIC ANTIBODY NEW
発明の概要(英語) Provided is a method whereby a high-affinity antibody can be efficiently produced with the use of a natural type protein as an antigen. Also provided is a method for obtaining information of an antigen-specific antibody derived exclusively from a blistered fish without being affected by microorganisms existing in the rearing environment. Information of a high-affinity antibody, i.e., an antigen-specific antibody is obtained with the use of a natural type protein as an antigen through the following steps, etc.: (A) a step for administering an antigen to a blistered fish and allowing the fish to produce an antibody; (B) a step for collecting the blister fluid containing the antibody from the fish treated in (A); and (C) a step for constructing an antigen-specific polyclonal antibody gene library from the blister fluid collected in (B). Thus, information of the antigen-specific antibody is obtained using primers, said primers being capable of amplifying only the gene of the antigen-specific antibody derived exclusively from the blistered fish but not amplifying genes of microorganisms existing in the rearing environment such as Propionibacterium acnes .
従来技術、競合技術の概要(英語) BACKGROUND ART
In the prior art, a variety of mammalian immune by an animal such as a mouse or a variety of antibodies such as phage display method is the method has been developed.However, these methods having a high affinity for the target protein of a naturally occurring antibody can be acquired, and a high purity target protein, and in the three-dimensional structure while maintaining the required conditions.In addition, the antibody by the immune system DNA are known in this method, this method is a dendritic cell can be expressed as in the native part of the protein when the target is effective.However, proteins cannot be expressed in a dendritic cell, a small amount of target protein as the expression of high affinity antibody was extremely difficult to acquire.Therefore, these known in the art depending on the method for antibody production, post-translational protein, sugar chain, fatty acid or the like is modified with the native protein as an antigen, such as drug discovery can be used to high-affinity antibodies have to be made efficiently.
Therefore, the present invention an administrator of the native protein as an antigen, and to efficiently manufacture high-affinity antibodies can be object of the present invention is to provide methods and.Then, in order to provide a method, an antibody specific for an antigen by fish having bubbles of information have been acquired.Having a raised by the bubbles because the fish in water, such as acne bacteria were present in the environment without being affected by the microorganisms, having only the fish and bubbles from the antigen-specific antibody may acquire the information of the object of the present invention.In addition, the inventors of the present invention, in Patent Document 1, and fish having bubbles by the object of the challenge, while making the state of the fish, the fish antibodies produced can be repeatedly obtained to develop a method of manufacturing an antibody.
  • 出願人(英語)
  • ※2012年7月以前掲載分については米国以外のすべての指定国
  • NATIONAL UNIVERSITY CORPORATION MIE UNIVERSITY
  • 発明者(英語)
  • TAMARU Yutaka
国際特許分類(IPC)
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