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TRANSGENIC MICROORGANISM AND USE THEREOF UPDATE 新技術説明会

外国特許コード F190009692
整理番号 (S2017-0573-N0)
掲載日 2019年1月23日
出願国 世界知的所有権機関(WIPO)
国際出願番号 2018JP016675
国際公開番号 WO 2018199112
国際出願日 平成30年4月24日(2018.4.24)
国際公開日 平成30年11月1日(2018.11.1)
優先権データ
  • 特願2017-086595 (2017.4.25) JP
発明の名称 (英語) TRANSGENIC MICROORGANISM AND USE THEREOF UPDATE 新技術説明会
発明の概要(英語) The purpose of the present invention is to provide: a microorganism which enables the production of muconic acid with good economic efficiency and high yield using a syringyllignin-rich biomass as a raw material; and a method for producing muconic acid using the microorganism. The purpose can be achieved by: a transgenic microorganism of which a host microorganism is a microorganism belonging to the family Sphingomonad , having a protocatechuic acid degrading enzyme gene on the chromosome thereof and capable of utilizing a syringyllignin-derived aromatic compound and in which the protocatechuic acid degrading enzyme gene on the chromosome is deleted, inserted catA gene can be expressed and inserted aroY gene or aroY gene and inserted kpdB gene can also be expressed; a method for producing muconic acid using the transgenic microorganism; and others.
従来技術、競合技術の概要(英語) BACKGROUND ART
Vascular plant cell wall components of lignin as the amorphous polymer substance is present, the complexity of the configuration of the fuzed unit-phenylpropane and, on the chemical structure containing a methoxy group are the features. Wood lignins are stuck to each other and to a plant cell, and serves to enhance the tissue, wood in an 18-36%, about 15-25% in the presence of the herbaceous. Therefore, to effectively utilize wood, and decomposition of lignin, an attempt to obtain useful compounds have been made various.
On the other hand, cis, cis - muconic acid (hereinafter, simply sometimes referred to as muconic acid.) Is, the carboxyl group and double bond in the molecule by 2 pieces, is a compound having high reactivity. Muconic acid as a starting material of the various known and muconic acid derivatives, for example, lactone, sulfone, polyamide, polyester, a thioester, additional polymers and the like. Such as muconic acid derivatives, various applications are known as having, for example, surfactants, flame retardants, UV light stabilizers, thermosetting plastics, may be used as coating agents.
In this way, muconic acid are, where subjected to a variety of applications, the lignin muconic acid can be produced, the reproduction of the resources is achieved, very useful. Therefore, a material derived from lignin or a lignin, muconic acid has been attempted a method of manufacturing. Particularly as such a method, using the bioconversion microorganisms have been studied.
For example, the following Non-Patent Document 1 (the entire document is incorporated as disclosed herein.) Is, Pseudomonas, putida ( Pseudomonas putida) as a host microorganism, a gene on a chromosome and gene pcaH pcaG (hereinafter, sometimes referred to as gene pcaHG with.) As well as gene catR, catB gene, the gene and catC catA gene disruption, and, inserted into the gene aroY catA gene, or gene ecdB aroY catA gene expression of a gene produced by transforming microorganisms, the microorganisms grown transformed glucose, caffeic acid then was muconic acid p- is described. In addition, the non-patent document 2 (described in the literature as disclosed herein all of which are hereby incorporated.) Is, , SYK-6 lines ( Sphingobiumspecies) Stanhylococcus vanillic acid and syringate can grow as the carbon source has been described.
  • 出願人(英語)
  • ※2012年7月以前掲載分については米国以外のすべての指定国
  • NAGAOKA UNIVERSITY OF TECHNOLOGY
  • HIROSAKI UNIVERSITY
  • 発明者(英語)
  • Masai Eiji
  • Naomi Uemura
  • TAKAHASHI KENJI
  • Kazuaki Sonomi
国際特許分類(IPC)
指定国 National States: AE AG AL AM AO AT AU AZ BA BB BG BH BN BR BW BY BZ CA CH CL CN CO CR CU CZ DE DJ DK DM DO DZ EC EE EG ES FI GB GD GE GH GM GT HN HR HU ID IL IN IR IS JO JP KE KG KH KN KP KR KW KZ LA LC LK LR LS LU LY MA MD ME MG MK MN MW MX MY MZ NA NG NI NO NZ OM PA PE PG PH PL PT QA RO RS RU RW SA SC SD SE SG SK SL SM ST SV SY TH TJ TM TN TR TT TZ UA UG US UZ VC VN ZA ZM ZW
ARIPO: BW GH GM KE LR LS MW MZ NA RW SD SL SZ TZ UG ZM ZW
EAPO: AM AZ BY KG KZ RU TJ TM
EPO: AL AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HR HU IE IS IT LT LU LV MC MK MT NL NO PL PT RO RS SE SI SK SM TR
OAPI: BF BJ CF CG CI CM GA GN GQ GW KM ML MR NE SN ST TD TG

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