Top > Search of International Patents > TRANSGENIC MICROORGANISM AND USE THEREOF

TRANSGENIC MICROORGANISM AND USE THEREOF meetings

Foreign code F190009692
File No. S2017-0573-C0
Posted date Jan 23, 2019
Country WIPO
International application number 2018JP016675
International publication number WO 2018199112
Date of international filing Apr 24, 2018
Date of international publication Nov 1, 2018
Priority data
  • P2017-086595 (Apr 25, 2017) JP
Title TRANSGENIC MICROORGANISM AND USE THEREOF meetings
Abstract The purpose of the present invention is to provide: a microorganism which enables the production of muconic acid with good economic efficiency and high yield using a syringyllignin-rich biomass as a raw material; and a method for producing muconic acid using the microorganism. The purpose can be achieved by: a transgenic microorganism of which a host microorganism is a microorganism belonging to the family Sphingomonad , having a protocatechuic acid degrading enzyme gene on the chromosome thereof and capable of utilizing a syringyllignin-derived aromatic compound and in which the protocatechuic acid degrading enzyme gene on the chromosome is deleted, inserted catA gene can be expressed and inserted aroY gene or aroY gene and inserted kpdB gene can also be expressed; a method for producing muconic acid using the transgenic microorganism; and others.
Outline of related art and contending technology BACKGROUND ART
Vascular plant cell wall components of lignin as the amorphous polymer substance is present, the complexity of the configuration of the fuzed unit-phenylpropane and, on the chemical structure containing a methoxy group are the features. Wood lignins are stuck to each other and to a plant cell, and serves to enhance the tissue, wood in an 18-36%, about 15-25% in the presence of the herbaceous. Therefore, to effectively utilize wood, and decomposition of lignin, an attempt to obtain useful compounds have been made various.
On the other hand, cis, cis - muconic acid (hereinafter, simply sometimes referred to as muconic acid.) Is, the carboxyl group and double bond in the molecule by 2 pieces, is a compound having high reactivity. Muconic acid as a starting material of the various known and muconic acid derivatives, for example, lactone, sulfone, polyamide, polyester, a thioester, additional polymers and the like. Such as muconic acid derivatives, various applications are known as having, for example, surfactants, flame retardants, UV light stabilizers, thermosetting plastics, may be used as coating agents.
In this way, muconic acid are, where subjected to a variety of applications, the lignin muconic acid can be produced, the reproduction of the resources is achieved, very useful. Therefore, a material derived from lignin or a lignin, muconic acid has been attempted a method of manufacturing. Particularly as such a method, using the bioconversion microorganisms have been studied.
For example, the following Non-Patent Document 1 (the entire document is incorporated as disclosed herein.) Is, Pseudomonas, putida ( Pseudomonas putida) as a host microorganism, a gene on a chromosome and gene pcaH pcaG (hereinafter, sometimes referred to as gene pcaHG with.) As well as gene catR, catB gene, the gene and catC catA gene disruption, and, inserted into the gene aroY catA gene, or gene ecdB aroY catA gene expression of a gene produced by transforming microorganisms, the microorganisms grown transformed glucose, caffeic acid then was muconic acid p- is described. In addition, the non-patent document 2 (described in the literature as disclosed herein all of which are hereby incorporated.) Is, , SYK-6 lines ( Sphingobiumspecies) Stanhylococcus vanillic acid and syringate can grow as the carbon source has been described.
Scope of claims (In Japanese)請求の範囲 [請求項1]
宿主微生物が染色体上にプロトカテク酸分解酵素遺伝子を有し、かつ、シリンギルリグニン由来の芳香族化合物を資化するスフィンゴモナド( Sphingomonad)科微生物であり、
染色体上にある該プロトカテク酸分解酵素遺伝子が欠失しており、
挿入されたcatA遺伝子を発現し、かつ、
挿入されたaroY遺伝子又はaroY遺伝子及びkpdB遺伝子を発現する、
形質転換微生物。

[請求項2]
挿入された前記aroY遺伝子、前記kpdB遺伝子及び前記catA遺伝子は、同一プロモーターの制御下にある、請求項1に記載の形質転換微生物。

[請求項3]
前記形質転換微生物は、挿入されたvanA遺伝子及びvanB遺伝子をさらに発現する、請求項1に記載の形質転換微生物。

[請求項4]
挿入された前記aroY遺伝子、前記kpdB遺伝子、前記catA遺伝子、前記vanA遺伝子及び前記vanB遺伝子は、同一プロモーターの制御下にある、請求項3に記載の形質転換微生物。

[請求項5]
前記プロトカテク酸分解酵素遺伝子が、ligA遺伝子、ligB遺伝子、pcaG遺伝子、pcaH遺伝子及びpraA遺伝子からなる群から選ばれる遺伝子である、請求項1に記載の形質転換微生物。

[請求項6]
前記宿主微生物が、スフィンゴビウム・スピーシーズ( Sphingobium species) SYK-6株である、請求項1に記載の形質転換微生物。

[請求項7]
p-ヒドロキシフェニルリグニン由来の芳香族化合物及び/又はグアイアシルリグニン由来の芳香族化合物と、シリンギルリグニン由来の芳香族化合物とを、請求項1~6のいずれか1項に記載の形質転換微生物に作用させることにより、ムコン酸を得る工程を含む、ムコン酸の製造方法。

[請求項8]
宿主微生物が染色体上にプロトカテク酸分解酵素遺伝子を有し、かつ、シリンギルリグニン由来の芳香族化合物を資化するスフィンゴモナド科微生物であり、かつ、
染色体上にある該プロトカテク酸分解酵素遺伝子が欠失している、
形質転換微生物。

[請求項9]
p-ヒドロキシフェニルリグニン由来の芳香族化合物及び/又はグアイアシルリグニン由来の芳香族化合物と、シリンギルリグニン由来の芳香族化合物とを、請求項8に記載の形質転換微生物に作用させることにより、プロトカテク酸を得る工程を含む、プロトカテク酸の製造方法。

  • Applicant
  • ※All designated countries except for US in the data before July 2012
  • NAGAOKA UNIVERSITY OF TECHNOLOGY
  • HIROSAKI UNIVERSITY
  • Inventor
  • MASAI, Eiji
  • KAMIMURA, Naofumi
  • TAKAHASHI, Kenji
  • SONOKI, Tomonori
IPC(International Patent Classification)

PAGE TOP

close
close
close
close
close
close