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TRANSGENIC MICROORGANISM CAPABLE OF PRODUCING MUCONIC ACID, AND USE THEREOF meetings

Foreign code F190009693
File No. (S2017-0570-N0),S2019-0034-N0
Posted date Jan 23, 2019
Country WIPO
International application number 2018JP016674
International publication number WO 2018199111
Date of international filing Apr 24, 2018
Date of international publication Nov 1, 2018
Priority data
  • P2017-086589 (Apr 25, 2017) JP
  • P2017-182566 (Sep 22, 2017) JP
Title TRANSGENIC MICROORGANISM CAPABLE OF PRODUCING MUCONIC ACID, AND USE THEREOF meetings
Abstract The purpose of the present invention is to provide: a microorganism which enables the production of muconic acid with good economic efficiency and high yield; and a method for producing muconic acid utilizing the microorganism. The purpose can be achieved by: a transgenic microorganism of which a host microorganism is a microorganism belonging to the genus Pseudomonas and has pcaH gene, pcaG gene, catA gene and catB gene on the chromosome thereof and in which the pcaH gene, the pcaG gene and the catB gene on the chromosome are deleted, and inserted pcaH gene and inserted pcaG gene can be expressed and inserted aroY gene is also expressed; a method for producing muconic acid using the transgenic microorganism; and others.
Outline of related art and contending technology BACKGROUND ART
Vascular plant cell wall components of lignin as the amorphous polymer substance is present, the complexity of the configuration of the fuzed unit-phenylpropane and, on the chemical structure containing a methoxy group are the features.Wood lignins are stuck to each other and to a plant cell, and serves to enhance the tissue, wood in an 18-36%, about 15-25% in the presence of the herbaceous.Therefore, to effectively utilize wood, and decomposition of lignin, an attempt to obtain useful compounds have been made various.
On the other hand, cis, cis - muconic acid (hereinafter, simply sometimes referred to as muconic acid.) Is, the carboxyl group and double bond in the molecule by 2 pieces, is a compound having high reactivity.Muconic acid as a starting material of the various known and muconic acid derivatives, for example, lactone, sulfone, polyamide, polyester, a thioester, additional polymers and the like.Such as muconic acid derivatives, various applications are known as having, for example, surfactants, flame retardants, UV light stabilizers, thermosetting plastics, may be used as coating agents.
In this way, muconic acid are, muconic acid derivatives are subjected to a variety of applications in the form of a result, lignin muconic acid can be produced, the reproduction of the resources is achieved, very useful.Therefore, a material derived from lignin or a lignin, muconic acid has been attempted a method of manufacturing.Particularly as such a method, the microorganisms of the bioconversion is used has been studied.
For example, the following Non-Patent Document 1 (described in the literature as disclosed herein all of which are hereby incorporated.) Is, Pseudomonas, putida ( Pseudomonas putida) as a host microorganism, a gene on a chromosome and gene pcaH pcaG (hereinafter, sometimes referred to as gene pcaHG with.) As well as gene catR, catB gene, the gene and catC catA gene disruption, and, inserted into the gene aroY catA gene, a gene or gene ecdB catA aroY gene expression to produce a transformed microorganisms, the microorganisms grown transformed glucose, caffeic acid then was muconic acid p- is described.
Scope of claims (In Japanese)請求の範囲 [請求項1]
宿主微生物が染色体上にpcaH遺伝子、pcaG遺伝子、catA遺伝子及びcatB遺伝子を有するシュードモナス( Pseudomonas)属微生物であり、
染色体上にある該pcaH遺伝子、該pcaG遺伝子及び該catB遺伝子が欠失しており、
挿入されたpcaH遺伝子及びpcaG遺伝子を発現し、かつ、
挿入されたaroY遺伝子を発現する、
形質転換微生物。

[請求項2]
挿入された前記aroY遺伝子と、挿入された前記pcaH遺伝子及び前記pcaG遺伝子とは、同一プロモーターの制御下にある、請求項1に記載の形質転換微生物。

[請求項3]
前記宿主微生物がさらに染色体上にpobA遺伝子、vanA遺伝子及びvanB遺伝子を有するシュードモナス属微生物である、請求項1に記載の形質転換微生物。

[請求項4]
前記宿主微生物がシュードモナス・プチダ( Pseudomonas  putida)である、請求項1に記載の形質転換微生物。

[請求項5]
p-ヒドロキシフェニルリグニン由来の芳香族化合物及び/又はグアイアシルリグニン由来の芳香族化合物を、請求項1~4のいずれか1項に記載の形質転換微生物に作用させることにより、ムコン酸を得る工程
を含む、ムコン酸の製造方法。

[請求項6]
溶存酸素濃度が1~13%である条件下で、p-ヒドロキシフェニルリグニン由来の芳香族化合物及び/又はグアイアシルリグニン由来の芳香族化合物を、請求項1~4のいずれか1項に記載の形質転換微生物に作用させることにより、ムコン酸を得る工程を含む、
ムコン酸の製造方法。

[請求項7]
aroY遺伝子とpcaH遺伝子及びpcaG遺伝子とを含む組換えベクター。

  • Applicant
  • ※All designated countries except for US in the data before July 2012
  • HIROSAKI UNIVERSITY
  • Inventor
  • SONOKI, Tomonori
  • MASAI, Eiji
  • KAMIMURA, Naofumi
IPC(International Patent Classification)

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