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METHOD FOR PROMOTING STORAGE MATERIAL PRODUCTION BY ALGA

Foreign code F190009761
File No. (S2017-0706-N0)
Posted date May 7, 2019
Country WIPO
International application number 2018JP017865
International publication number WO 2018207804
Date of international filing May 9, 2018
Date of international publication Nov 15, 2018
Priority data
  • P2017-093581 (May 10, 2017) JP
Title METHOD FOR PROMOTING STORAGE MATERIAL PRODUCTION BY ALGA
Abstract [Problem] To provide a method, whereby an alga can be continuously cultured using a low-cost and simple means for inducing lipid synthesis, in order to efficiently utilize the alga as a biomass.
[Solution] A method for promoting the production of a storage material by an alga, said method comprising a step for continuously culturing and proliferating the alga under light irradiation conditions in a culture solution containing an oxystress inducing agent.
Outline of related art and contending technology BACKGROUND ART
To suppress generation of carbon dioxide as a clean energy source, fuel derived from biomass (biofuel) is expected. Biofuels, algae, animal oils, soy, corn, oil and other cereal or the like produced using the various resources may be, the algae are, for each of the grain or the like to cause a problem of an increase in contrast they are not concerned with the food, is easy to culture, the type of the storage material and the production efficiency can be adjusted, are of particular interest as next generation energy source. So far, such as fats and sugars, increase the production capacity of the storage material of the algae a variety of methods have been studied.
Algae, nutritional deficiencies including nitrogen can be accumulated by the fat is known, the growth of algae is deteriorated by the nutritional deficiencies. That is, to suppress the production of biomass such as stress state, inducing the accumulation of fat in the algae is considered. Biomass production or both of the amount of fat and 1 as one means, algae biomass in optimized conditions to increase after culturing algae, fat accumulation is changed to the stress conditions to maximize the two-stage culture is being performed (for example, see Non-Patent Document 1). Such as a nitrogen starvation of the stress state, the active oxygen species in the growth of algae (ROS) results in an increase, the oxidation stress accumulation of fat in the algae is a mediator has been suggested (for example, see Non-Patent Document 2).
The inventors of the present invention, the leaves of green algae in the body by increasing the concentration of glutathione, nutrient limitation without performing the step, can improve the productivity of the biomass was found to be (for example, see Patent Document 1). The mechanism in its metabolism, oxidative stress and the productivity of the storage material and the relation between. Oxidative stress and lipid content to provide the algae is increased have been reported to another device (for example, see Patent Document 2 and 3).
Scope of claims (In Japanese)請求の範囲 [請求項1]
 藻類による貯蔵物質の産生を促進する方法であって、
 酸化ストレス誘導剤を含む培養液中で、前記藻類を、光照射条件下にて継続的に培養し増殖させる工程を含む、方法。

[請求項2]
 前記酸化ストレス誘導剤が、前記藻類の細胞内のグルタチオン濃度を変化させる剤である請求項1に記載の方法。

[請求項3]
 前記酸化ストレス誘導剤が、パラコート、過酸化水素及び次亜塩素酸塩からなる群より選択される剤であり、前記藻類の増殖を維持しうる濃度で前記培養液に含まれる請求項1又は2に記載の方法。

[請求項4]
 前記貯蔵物質が、炭素密度の高い有機物質である、請求項1~3のいずれか一項に記載の方法。

[請求項5]
 前記貯蔵物質が、脂質及び/又は糖質である、請求項1~4のいずれか一項に記載の方法。

[請求項6]
 請求項1~5のいずれか一項に記載の方法を用いて藻類を培養する工程と、
 前記培養液の少なくとも一部を回収する工程と、を含む、藻類による貯蔵物質の製造方法。

[請求項7]
 請求項1~5のいずれか一項に記載の方法を用いて藻類を培養する工程と、
 前記培養液の少なくとも一部を回収する工程と、を含む、藻類を含むバイオマスの製造方法。

  • Applicant
  • ※All designated countries except for US in the data before July 2012
  • KEIO UNIVERSITY
  • Inventor
  • ITO Takuro
  • OGAWA KENICHI
  • NISHIKAWA Masanobu
IPC(International Patent Classification)
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