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KIT FOR DETECTING OR MEASURING ANTIGEN NEW

外国特許コード F190009774
整理番号 S2016-1193-C0
掲載日 2019年5月7日
出願国 世界知的所有権機関(WIPO)
国際出願番号 2018JP001186
国際公開番号 WO 2018147018
国際出願日 平成30年1月17日(2018.1.17)
国際公開日 平成30年8月16日(2018.8.16)
優先権データ
  • 特願2017-021164 (2017.2.8) JP
発明の名称 (英語) KIT FOR DETECTING OR MEASURING ANTIGEN NEW
発明の概要(英語) The purpose of the present invention is to develop a kit capable of detecting or measuring an antigen at high sensitivity without requiring an immobilization step or a washing step. The present invention provides a kit for detecting or measuring an antigen, the kit comprising: a conjugate composed of a polypeptide including an antibody light chain variable region and a polypeptide including an antibody heavy chain variable region; and a fragment of protein M marked with a fluorescent pigment, wherein the fragment of protein M is a fragment having the ability to bond with the conjugate.
従来技術、競合技術の概要(英語) BACKGROUND ART
Current, are becoming increasingly important in clinical diagnosis and immune measuring method is a measurement technique has been. Is adopted to avoid individual immunoassay, the sensitivity, as well as improve specificity, rapid measurement, and also simplification has been an important factor. Immunoassays in the current mainstream, a sandwich method for detecting protein biomarkers, the conflict can be detected by a low molecular weight used as a measurement principle of the method. However both of which, after the reaction and washing several times in the main measuring the enzymatic activity using a label enzyme immunoassay method in many cases, few hours of labor and the measurement is a problem that it takes. In contrast, the measurement sample by mixing a reaction reagent, homogeneous immunoassay detection have been developed.
The present inventors have, in recent years, in such a homogeneous immunoassay can be used as a measuring device with high sensitivity quickly binds to the antigen antibody Quenchbody shines successful construction of a (Q-body), such as paper and patent application publication (WO2011/061944,WO2013/065314,R. Abe et al., J. Am. Chem. Soc., 2011, 133 (43), 17386-17394) been done. Q-body is, in the vicinity of the antigen binding site of a particular 1 location 2 of the location, via a short linker such as a fluorescent dye TAMRA-labeled modified antibody and fluorescent, dye (mainly tryptophan) amino acids of an antibody interacting with, the quenched state (extinction), the antigen is added to the light-off is released. However, conventionally in the production of the antibody gene cloning is essential to construct was a problem that it takes time and cost.
In order to solve this problem, recently, the present inventors, paying attention to the fact that PAxPG (J. Dong et al., J. Biosci. Bioeng., 2015, 120, 504-509) antibody-bound protein, the protein with a fluorescent dye and the linker is coupled via a fluorescent probe, a mixture of an antibody, the same Q-body, is released by the addition of the antigen in the production of the complex quencher was successful (non-patent document 1).
  • 出願人(英語)
  • ※2012年7月以前掲載分については米国以外のすべての指定国
  • TOKYO INSTITUTE OF TECHNOLOGY
  • 発明者(英語)
  • UEDA, Hiroshi
  • OHMURO, Yuki
  • MIYAKE, Chihiro
  • TSUKAHARA, Tomoya
国際特許分類(IPC)
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