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METHOD FOR MAKING BIOLOGICAL TISSUE TRANSPARENT AND REAGENT FOR SAME

外国特許コード F190009778
整理番号 (S2017-0882-N0)
掲載日 2019年5月7日
出願国 世界知的所有権機関(WIPO)
国際出願番号 2018JP025239
国際公開番号 WO 2019009300
国際出願日 平成30年7月3日(2018.7.3)
国際公開日 平成31年1月10日(2019.1.10)
優先権データ
  • 特願2017-132787 (2017.7.6) JP
発明の名称 (英語) METHOD FOR MAKING BIOLOGICAL TISSUE TRANSPARENT AND REAGENT FOR SAME
発明の概要(英語) Provided is a method for quickly making biological tissue transparent. A method for making biological tissue transparent, the method being characterized by including: a step for impregnating biological tissue with water-soluble ethylenic unsaturated monomers before, during, or after the fixing of the biological tissue by a fixing agent, the water-soluble ethylenic unsaturated monomers including at least a water-soluble ethylenic unsaturated monomer that has an ionic leaving group; a step for forming a hydrogel by polymerizing the water-soluble ethylenic unsaturated monomers inside the biological tissue post-fixing; and a step for removing lipids from the biological tissue post-fixing.
従来技術、競合技術の概要(英語) BACKGROUND ART
Fluorescence imaging, diagnosis of diseases or in vivo biological phenomenon at the time of 1 as one of useful tools have been used, in the conventional technique, a tissue (for example the slice) as a two-dimensional image of the vicinity of the surface, it is difficult to obtain a three-dimensional image. In general, the biological tissue is a protein, lipid, nucleic acid or the like is configured, for each of these substances may have different refractive indexes, a refractive index thereof is in the living tissue and the non-uniform, and thus light do not pass through living tissue, living tissue can be observed from the inside of the surface is not possible.
Therefore, the refractive index of the entire living tissue, protein main component thereof is brought close to the scattering of light in the living tissue as a result suppress, a biological tissue has been developed a technique to be transparent. Chung et al. 'CLARITY' method, light scattering and refractive index of the non-uniform major lipid, protein polyacrylamide hydrogels were fixed are removed by the electrophoresis, liquid tissue, protein a solution close to that of the refractive index can be substituted by a, tissue in the art to be transparent (non-patent document 1, Patent Document 1).
CLARITY method, tissue destruction is likely to occur by electrophoresis for a problem in that, instead of the electrophoresis method of removing lipids gradually by shaking has been developed (Non-Patent Document 2, Patent Document 1, 2). However, in this method, tissue destruction hardly occurs on the other hand, the removal by shaking (2-3 weeks) for a long time was a problem that it takes.
  • 出願人(英語)
  • ※2012年7月以前掲載分については米国以外のすべての指定国
  • OSAKA PREFECTURE UNIVERSITY
  • 発明者(英語)
  • KOJIMA, Chie
  • MATSUMOTO, Akikazu
国際特許分類(IPC)
指定国 National States: AE AG AL AM AO AT AU AZ BA BB BG BH BN BR BW BY BZ CA CH CL CN CO CR CU CZ DE DJ DK DM DO DZ EC EE EG ES FI GB GD GE GH GM GT HN HR HU ID IL IN IR IS JO JP KE KG KH KN KP KR KW KZ LA LC LK LR LS LU LY MA MD ME MG MK MN MW MX MY MZ NA NG NI NO NZ OM PA PE PG PH PL PT QA RO RS RU RW SA SC SD SE SG SK SL SM ST SV SY TH TJ TM TN TR TT TZ UA UG US UZ VC VN ZA ZM ZW
ARIPO: BW GH GM KE LR LS MW MZ NA RW SD SL SZ TZ UG ZM ZW
EAPO: AM AZ BY KG KZ RU TJ TM
EPO: AL AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HR HU IE IS IT LT LU LV MC MK MT NL NO PL PT RO RS SE SI SK SM TR
OAPI: BF BJ CF CG CI CM GA GN GQ GW KM ML MR NE SN ST TD TG

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