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SAMPLE OBSERVATION METHOD, SAMPLE OBSERVATION DEVICE, AND MICROSCOPE NEW

外国特許コード F190009785
整理番号 (S2017-0956-N0)
掲載日 2019年5月7日
出願国 世界知的所有権機関(WIPO)
国際出願番号 2018JP031302
国際公開番号 WO 2019039581
国際出願日 平成30年8月24日(2018.8.24)
国際公開日 平成31年2月28日(2019.2.28)
優先権データ
  • 特願2017-162635 (2017.8.25) JP
発明の名称 (英語) SAMPLE OBSERVATION METHOD, SAMPLE OBSERVATION DEVICE, AND MICROSCOPE NEW
発明の概要(英語) [Problem] The purpose of the present invention is to provide a transmission-type sample observation method and a transmission-type microscope that enable spatial spectrum analysis and clarification of an image of a sample even when the microscope has an adjustable-focal-point optical system.
[Solution] In a sample observation method and sample observation device according to the present invention: observations are made in certain camera-side pixels; a large-region component (B) and a large-region component (C) that are present when an observation point is positioned in a dark-illumination part are removed from a direct component (A), the large-region component (B), and the large-region component (C) that are present when the observation point is positioned in a bright-illumination part, the direct component (A) being formed from observation-point light that is single-scattered from the observation point when focus has been achieved, the large-region component (B) being formed from light that is single-scattered from a non-focused point, and the large-region component (C) being formed from multiple-scattered light; and the direct component (A) is thereby extracted by using the difference between a focused state that can be used as pattern illumination of the bright- and dark-illumination parts and a non-focused state in which the pattern illumination is uniform.
従来技術、競合技術の概要(英語) BACKGROUND ART
A transmission type optical microscope, biomedical, food safety, and many other applications are widely used. When a sample is observed with a microscope, the optical image sensor before reaching the small particles in the sample to change the direction of the strikes. As a result, the image sensor, the sum of the scattered light passing through the different paths will be captured. The scattering of light is, the image observed by the indistinct. (B) is shown in Fig. 1, the micro-meter (Fig. 1 (a) ) was placed on top of the scattering transmission electron microscope observation of the image. In this way the scattering of light is to blur the image.
The obscuration of the image due to scattering, it is a big problem in biomedical image is formed. For example, in the spatio-spectral analysis of biological tissue, the absorption coefficient is at a particular point must be accurately measured. However, the signal is measured, and the specific point of the light scattered from different points may include, different points within the tissue and scattered light to the information. At a particular point of the biological tissue is measured accurately, a particular direct light from the other scattered light from the point of separation is essential.
(Computational Photography) in the field of computational photography method, a sample of the global component from the reflected light of the light-reflection is removed, the specular reflection light and diffuse reflected light and the direct component is extracted have been proposed several methods. For example, in Non-Patent Document 1, discloses the use of high-frequency illumination. The illumination is high, the high-frequency spatial patterns may be applied to the same location in the global component and the direct component can be separated (for example, see Non-Patent Document 3-8.). On the other hand, in Non-Patent Document 2, Non-Patent Document 1 is different from the reflective method, a translucent object such as the separation of the components in the transmission observation (observation point passes through the observation point single scattering light directly and the other point is scattered at other points a single light scattering and multiple scattering light separation of the global component) of a transmissive technique is introduced. This approach, the high frequency of the parallel light using the illumination light to be observed are to be transmitted. Therefore, a special lens in the non-patent document 2 (telecentric lens) and the high-frequency illumination using collimated light, the parallel light to be observed has been configured.
  • 出願人(英語)
  • ※2012年7月以前掲載分については米国以外のすべての指定国
  • RESEARCH ORGANIZATION OF INFORMATION AND SYSTEMS
  • 発明者(英語)
  • SHIMANO, Mihoko
  • BISE, Ryoma
  • ZHENG, Yinqiang
  • SATO, Imari
国際特許分類(IPC)
指定国 National States: AE AG AL AM AO AT AU AZ BA BB BG BH BN BR BW BY BZ CA CH CL CN CO CR CU CZ DE DJ DK DM DO DZ EC EE EG ES FI GB GD GE GH GM GT HN HR HU ID IL IN IR IS JO JP KE KG KH KN KP KR KW KZ LA LC LK LR LS LU LY MA MD ME MG MK MN MW MX MY MZ NA NG NI NO NZ OM PA PE PG PH PL PT QA RO RS RU RW SA SC SD SE SG SK SL SM ST SV SY TH TJ TM TN TR TT TZ UA UG US UZ VC VN ZA ZM ZW
ARIPO: BW GH GM KE LR LS MW MZ NA RW SD SL SZ TZ UG ZM ZW
EAPO: AM AZ BY KG KZ RU TJ TM
EPO: AL AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HR HU IE IS IT LT LU LV MC MK MT NL NO PL PT RO RS SE SI SK SM TR
OAPI: BF BJ CF CG CI CM GA GN GQ GW KM ML MR NE SN ST TD TG
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