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MODIFIED COLLAGEN PROTEIN AND APPLICATION OF SAME

外国特許コード F190009865
整理番号 (S2018-0110-N0)
掲載日 2019年7月26日
出願国 世界知的所有権機関(WIPO)
国際出願番号 2018JP042181
国際公開番号 WO 2019098246
国際出願日 平成30年11月14日(2018.11.14)
国際公開日 令和元年5月23日(2019.5.23)
優先権データ
  • 特願2017-219515 (2017.11.14) JP
発明の名称 (英語) MODIFIED COLLAGEN PROTEIN AND APPLICATION OF SAME
発明の概要(英語) In order to develop tools and methods useful in a variety of applications, including the research and development of medical treatments which involve the modification of collagen protein and use of the same, the present invention provides a modified collagen protein expressed in a transformed cell and capable of forming collagen fibers outside of said cell, wherein the transformation is performed by introducing, into the cell, into the cell, polynucleotides coding the modified collagen protein.
従来技術、競合技術の概要(英語) BACKGROUND ART
Tissue fibrosis is, accompanied by inflammation and wound healing damage becomes excessive, the tissue is generated to replace the normal tissue, causing deterioration in the functioning of organs and tissues (for example, in the lung interstitial pneumonia, infection, pneumonia etc. (smoking can cause); in the liver, liver or the like (viral infection, alcohol and the like can cause); in the kidney is, diabetes stage or the like; in the heart, such as heart failure; or post-operative adhesions and the like). Particularly likely to occur on the end of a variety of diseases, which is the main cause of organ failure. The analysis of these occurrences, the experimental animal anatomy (i.e., invasive method) in a series of analysis is necessary.
In the prior art, N or C terminus is at the end of the collagenous proteins are secreted outside when the cutting, is removed, a long central portion of the collagen polypeptide (amount of the helix portion 3) for only contributes to the formation of fibers, such as N or C terminus is added at the end of imaging is a fluorescent protein, cannot be detected only in the cells transporting, after cutting out the cells at the time of the collagen fibers was impossible to visualize.
In addition, fibers 3 are involved in the formation amount of the helix portion and the stored portion among species, structurally stable, this portion of the other protein it was difficult to insert. (Fig. 1; Non-Patent Document 1: Prockop DJ et al., New Engl J Med, Vol.301, 13-23, 1979) therefore, typical fusion protein in the method of labeling may be difficult.
Fibrosis is a component of Type I, III, V, among collagen XI, XI collagen TypeV and exceptionally has N-terminal domain is not cut, in particular, such as a set of Type I collagen when forming thicker fibers, fibrillar collagen Type V N-terminal domain and the outside of the considered (Fig. 2; Non-Patent Document 2: Simone M. Smith and David E. Birk, Exp Eye Res Vol.98, 105-106, 2012)
Human, and human collagen expression vector, the manufacturing method of the collagen, in the patent literature have been reported (Patent Document 1: Japanese Patent Application Laid-Open 08-023979). In this document, it is the full-length genes collagen type 3 human integrated into the vector expression has been reported, the correct protein is synthesized on the electrophoresis as is, the original form of the collagen fibers outside of the cell whether or not will be described. sf9 Cells primarily produce other types of collagen is not, the cell with the correct formation of collagen fibers will not be able to appear. In addition, the probe and its use in the method for tissue analysis report may be in the literature (Japanese Patent Laid-Open 2:WO2012/124338). In this patent, collagen-binding domain of collagenase and a fluorescent protein is added to the probe is disclosed.
  • 出願人(英語)
  • ※2012年7月以前掲載分については米国以外のすべての指定国
  • UNIVERSITY OF TSUKUBA
  • 発明者(英語)
  • MIWA Yoshihiro
  • KIJIMA Junko
国際特許分類(IPC)
指定国 National States: AE AG AL AM AO AT AU AZ BA BB BG BH BN BR BW BY BZ CA CH CL CN CO CR CU CZ DE DJ DK DM DO DZ EC EE EG ES FI GB GD GE GH GM GT HN HR HU ID IL IN IR IS JO JP KE KG KH KN KP KR KW KZ LA LC LK LR LS LU LY MA MD ME MG MK MN MW MX MY MZ NA NG NI NO NZ OM PA PE PG PH PL PT QA RO RS RU RW SA SC SD SE SG SK SL SM ST SV SY TH TJ TM TN TR TT TZ UA UG US UZ VC VN ZA ZM ZW
ARIPO: BW GH GM KE LR LS MW MZ NA RW SD SL SZ TZ UG ZM ZW
EAPO: AM AZ BY KG KZ RU TJ TM
EPO: AL AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HR HU IE IS IT LT LU LV MC MK MT NL NO PL PT RO RS SE SI SK SM TR
OAPI: BF BJ CF CG CI CM GA GN GQ GW KM ML MR NE SN ST TD TG
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