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Method and kit for detecting pathogenic microorganism NEW

外国特許コード F190009868
整理番号 AF19-14TW
掲載日 2019年7月29日
出願国 台湾
出願番号 106130237
公報番号 201812297
出願日 平成29年9月5日(2017.9.5)
公報発行日 平成30年4月1日(2018.4.1)
優先権データ
  • 特願2016-172515 (2016.9.5) JP
  • 特願2017-099579 (2017.5.19) JP
発明の名称 (英語) Method and kit for detecting pathogenic microorganism NEW
発明の概要(英語) Provided is a method for detecting pathogenic microorganisms in a biological sample, which is a technique that can be used to perform high-sensitivity detection of pathogenic microorganisms, such as influenza virus, etc., the method including: an introducing step for introducing a hydrophilic solvent that contains the biological sample and substances that serve as substrates for reactions involving enzymes that are present on surfaces of the pathogenic microorganisms or in the interiors thereof into a space between a lower-layer section in which a plurality of accommodating sections that can accommodate the pathogenic microorganisms are formed and an upper-layer section that faces a surface in which the accommodating sections are formed in the lower-layer section; an encapsulating step for introducing a hydrophobic solvent into the space and forming, in the accommodating sections, droplets of the hydrophilic solvent that are coated by the hydrophobic solvent enveloping the pathogenic microorganisms and the substances; and a detecting step for optically detecting reaction products that are generated as a result of the reactions between the enzymes and the substances in the droplets, wherein the hydrophilic solvent has a pH value that is greater than the acid dissociation constant (pKa) of the reaction products.
特許請求の範囲(英語) [claim1]
1. A methods, it is examines the self inductance catching an illness original nature microorganism the object or has in the biological sample that the suspicion infection's object separates this pathogenicity microorganism's method, its includes: Inducts the step, it is in may accept the above pathogenicity microorganism a plural number admission section because of the lower level department that having the hydrophobic surface's sidewall each other was separated to form, and in this lower level department forms this admission section facing to the space between upper departments, inducts water affinity solvent the step, but this water affinity solvent will include this biological sample and will become the use to exist in the surface or internal enzyme's this pathogenicity microorganism matrix of response material; Seals into the step, it is inducts the hydrophobic solvent in this space, but in this admission section, forms after the hydrophobic solvent superposition and contains this pathogenicity microorganism and this material the step of bubble of water affinity solvent; The examination step, it is optics examine in this bubble because of this enzyme with the step that of reaction product the response of this material produces; And this water affinity solvent has the acid hydrolysis of this reaction product to leave the constant (pKa) also big pH value.

[claim2]
2. Like request 1 method, this pathogenicity microorganism is the influenza virus, this enzyme for the nerve amine saccharinic acid enzyme, this material is 4- methyl umbrella type bases - α-D - nerve amine saccharinic acid (4-Methylumbelliferyl-N-acetyl-α-D-neuraminic acid), this reaction product is 4- methyl umbrella type colored lactones.

[claim3]
3. 1 kinds of suites, it is uses to examine the self inductance catching an illness original nature microorganism the object or has in the biological sample that the suspicion infection's object separates this pathogenicity microorganism's suite, its includes: The array, its has to be possible to accept this pathogenicity microorganism a plural number admission section, and to this lower level department in forms the surface gap space that because of the lower level department that having the hydrophobic surface's sidewall each other was separated to form this admission section forms, but to approaching upper department; Will become the use to exist in the surface or internal enzyme's this pathogenicity microorganism matrix of response material; Has uses this enzyme to leave the constant (pKa) also big pH value with acid hydrolysis that of the reaction product the response of this material produces the water affinity solvent; And hydrophobic solvent.

[claim4]
4. 1 methods, it is in makes the contact surface contact with the hydrophobic solvent in the water affinity solvent, makes the enzyme, attending to become the material that the matrix that of response uses this enzyme responded, but the method of examination reaction product, this water affinity solvent has the acid hydrolysis of this reaction product to leave the constant (pKa) also big pH value.

[claim5]
5. Like request 4 method, this water affinity solvent includes the pathogenicity microorganism, this enzyme to exist has the matrix to shut off the active enzyme in the surface or the interior this pathogenicity microorganism, this material is the colored matrix, optics examination because of using this enzyme cut-off of the colored matrix the reaction product of production.

[claim6]
6. Like request 5 method, this pathogenicity microorganism is the influenza virus, this enzyme for the nerve amine saccharinic acid enzyme, this colored matrix is 4- methyl umbrella type bases - α-D - nerve amine saccharinic acid (4-Methylumbelliferyl-N-acetyl-α-D-neuraminic acid), this reaction product is 4- methyl umbrella type colored lactones.

[claim7]
7. Like request item 5 or 6 methods, this water affinity solvent includes from infecting this pathogenicity microorganism's object or has the biological sample that the suspicion infection's object separates.
  • 出願人(英語)
  • JAPAN SCIENCE AND TECHNOLOGY AGENCY
  • 発明者(英語)
  • NOJI HIROYUKI
  • TABATA KAZUHITO
国際特許分類(IPC)
参考情報 (研究プロジェクト等) CREST Creation of Nanosystems with Novel Functions through Process Integration AREA
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