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METHOD FOR PRODUCING MEMBRANE PROTEIN NEW_EN achieved

Foreign code F190009892
File No. 07366-EP
Posted date Aug 26, 2019
Country EPO
Application number 08846826
Gazette No. 2213747
Date of filing Nov 5, 2008
Gazette Date Aug 4, 2010
International application number JP2008070116
International publication number WO2009060857
Date of international filing Nov 5, 2008
Date of international publication May 14, 2009
Priority data
  • P2007-286899 (Nov 5, 2007) JP
Title METHOD FOR PRODUCING MEMBRANE PROTEIN NEW_EN achieved
Abstract A method is provided for producing a membrane protein folded to its native structure or active structure in a lipid disc or a liposome. The method comprises the following steps: (a) preparing a reaction solution for cell-free protein synthesis containing a polynucleotide encoding a membrane protein, a steroidal detergent, and a phospholipid, wherein the steroidal detergent is contained at a concentration higher than its critical micelle concentration; (b) decreasing the concentration of said steroidal detergent in the reaction solution; and (c) synthesizing the membrane protein simultaneously with formation of a lipid disk or liposome into which the synthesized membrane protein is integrated.
  • Applicant
  • RIKEN
  • Inventor
  • YOKOYAMA, SHIGEYUKI
  • SHIMONO, KAZUMI
  • SHIROUZU, MIKAKO
  • GOTO, MIE
IPC(International Patent Classification)

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