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METHOD FOR CONSTRUCTING RECOMBINANT BACTERIUM FOR PRODUCING NON-NATIVE PROTEIN, AND UTILIZATION OF SAME NEW_EN achieved

Foreign code F190009893
File No. 07783-EP
Posted date Aug 26, 2019
Country EPO
Application number 11795797
Gazette No. 2584037
Gazette No. 2584037
Date of filing Jun 16, 2011
Gazette Date Apr 24, 2013
Gazette Date Aug 10, 2016
International application number JP2011063778
International publication number WO2011158895
Date of international filing Jun 16, 2011
Date of international publication Dec 22, 2011
Priority data
  • P2010-137635 (Jun 16, 2010) JP
  • P2011-047663 (Mar 4, 2011) JP
Title METHOD FOR CONSTRUCTING RECOMBINANT BACTERIUM FOR PRODUCING NON-NATIVE PROTEIN, AND UTILIZATION OF SAME NEW_EN achieved
Abstract The present invention provides a novel method of producing a recombinant bacterium for production of a non-natural protein, including: (1) expressing tRNA in a bacterium, which tRNA recognizes UAG codon; (2) expressing an aminoacyl-tRNA synthetase in the bacterium, which aminoacyl-tRNA synthetase acylates the tRNA with a non-natural amino acid or an ±-hydroxy acid; (3) (i) introducing a DNA construct into the bacterium, which DNA construct is for expressing, in the absence of a release factor for terminating translation at UAG codon, a function of at least one gene selected from the group consisting of genes each of which loses its function when a gene that codes for the release factor is defective and/or introducing an alteration into said at least one gene in a chromosome of the bacterium, which alteration is for expressing the function of said at least one gene in the absence of the release factor; and (4) causing the gene that codes for the release factor in the bacterium to be defective.
  • Applicant
  • RIKEN
  • Inventor
  • YOKOYAMA, SHIGEYUKI
  • MUKAI, TAKAHITO
  • SAKAMOTO, KENSAKU
  • MATSUMOTO, AKIKO
IPC(International Patent Classification)

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