Top > Search of International Patents > Method of using cell-free protein synthesis to produce a membrane protein

Method of using cell-free protein synthesis to produce a membrane protein NEW_EN achieved

Foreign code F190009902
File No. 07366-US
Posted date Aug 26, 2019
Country United States of America
Application number 74157508
Gazette No. 20100291189
Gazette No. 8226976
Date of filing Nov 5, 2008
Gazette Date Nov 18, 2010
Gazette Date Jul 24, 2012
International application number JP2008070116
International publication number WO2009060857
Date of international filing Nov 5, 2008
Date of international publication May 14, 2009
Priority data
  • P2007-286899 (Nov 5, 2007) JP
Title Method of using cell-free protein synthesis to produce a membrane protein NEW_EN achieved
Abstract A method is provided for producing a membrane protein folded to its native structure or active structure in a lipid disc or a liposome. The method comprises: (a) preparing a reaction solution for cell-free protein synthesis containing a polynucleotide encoding a membrane protein, a steroidal detergent, and a phospholipid, wherein the steroidal detergent is contained at a concentration higher than its critical micelle concentration; (b) decreasing the concentration of said steroidal detergent in the reaction solution; and (c) synthesizing the membrane protein simultaneously with formation of a lipid disk or liposome into which the synthesized membrane protein is integrated.
  • Inventor, and Inventor/Applicant
  • Yokoyama; Shigeyuki
  • Shimono; Kazumi
  • Shirouzu; Mikako
  • Goto; Mie
  • RIKEN
IPC(International Patent Classification)

PAGE TOP

close
close
close
close
close
close