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METHOD FOR CONSTRUCTING RECOMBINANT BACTERIUM FOR PRODUCING NON-NATIVE PROTEIN, AND UTILIZATION OF SAME NEW 実績あり

外国特許コード F190009905
整理番号 07783-US
掲載日 2019年8月26日
出願国 アメリカ合衆国
出願番号 201113704391
公報番号 20130095524
公報番号 9340790
出願日 平成23年6月16日(2011.6.16)
公報発行日 平成25年4月18日(2013.4.18)
公報発行日 平成28年5月17日(2016.5.17)
国際出願番号 JP2011063778
国際公開番号 WO2011158895
国際出願日 平成23年6月16日(2011.6.16)
国際公開日 平成23年12月22日(2011.12.22)
優先権データ
  • 特願2010-137635 (2010.6.16) JP
  • 特願2011-047663 (2011.3.4) JP
発明の名称 (英語) METHOD FOR CONSTRUCTING RECOMBINANT BACTERIUM FOR PRODUCING NON-NATIVE PROTEIN, AND UTILIZATION OF SAME NEW 実績あり
発明の概要(英語) The present invention provides a novel method of producing a recombinant bacterium for production of a non-natural protein, including: (1) expressing tRNA in a bacterium, which tRNA recognizes UAG codon; (2) expressing an aminoacyl-tRNA synthetase in the bacterium, which aminoacyl-tRNA synthetase acylates the tRNA with a non-natural amino acid or an .alpha.-hydroxy acid; (3) (i) introducing a DNA construct into the bacterium, which DNA construct is for expressing, in the absence of a release factor for terminating translation at UAG codon, a function of at least one gene selected from the group consisting of genes each of which loses its function when a gene that codes for the release factor is defective and/or introducing an alteration into said at least one gene in a chromosome of the bacterium, which alteration is for expressing the function of said at least one gene in the absence of the release factor; and (4) causing the gene that codes for the release factor in the bacterium to be defective.
  • 発明者/出願人(英語)
  • Yokoyama; Shigeyuki
  • Mukai; Takahito
  • Sakamoto; Kensaku
  • Matsumoto; Akiko
  • RIKEN
国際特許分類(IPC)

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