TOP > 外国特許検索 > Method for detecting bilirubin using a fluorescent protein

Method for detecting bilirubin using a fluorescent protein UPDATE 実績あり

外国特許コード F190009911
整理番号 08118-US
掲載日 2019年8月26日
出願国 アメリカ合衆国
出願番号 201414771060
公報番号 20160009771
公報番号 9957308
出願日 平成26年2月28日(2014.2.28)
公報発行日 平成28年1月14日(2016.1.14)
公報発行日 平成30年5月1日(2018.5.1)
国際出願番号 JP2014055160
国際公開番号 WO2014133158
国際出願日 平成26年2月28日(2014.2.28)
国際公開日 平成26年9月4日(2014.9.4)
優先権データ
  • 特願2013-040097 (2013.2.28) JP
  • 2014JP55160 (2014.2.28) WO
発明の名称 (英語) Method for detecting bilirubin using a fluorescent protein UPDATE 実績あり
発明の概要(英語) In order to provide a novel fluorescent protein and use thereof, the polypeptide according to the present invention has fluorescent properties in the presence of bilirubin and includes (1) the amino acid sequence of SEQ ID NO: 1, (2) an amino acid sequence having, for example, substitution of 1 to 21 amine acids in the amino acid sequence of SEQ ID NO: 1, (3) an amino acid sequence having 85% or more sequence identity to the amino acid sequence of SEQ ID NO: 1, or (4) the amino acid sequence encoded by a polynucleotide that hybridizes with a polynucleotide consisting of a sequence complementary to the polynucleotide encoding the polypeptide according to the amino acid sequence in (1) under a stringent condition.
従来技術、競合技術の概要(英語) BACKGROUND ART
Fluorescent proteins, such as green fluorescent protein (GFP), are indispensable as a tool for visualizing cells, tissue, biological individuals, and so on.
Most of fluorescent proteins have been isolated from invertebrates such as coral, sea anemones, and arthropods. However, for example, Non Patent Literatures 1 and 2 and Patent Literatures 1 and 2 report that a vertebrate, Japanese eel (Anguilla japonica), has a fluorescent protein.
特許請求の範囲(英語) [claim1]
1. A method for detecting bilirubin in a subject, the method comprising:
contacting a sample from the subject with a polypeptide, wherein the polypeptide has fluorescent activity upon binding to bilirubin; and
detecting fluorescence emitted by the polypeptide after the contacting step,
wherein the presence of fluorescence emission detects bilirubin in the subject, and
wherein the polypeptide is selected from the group consisting of;
(1) a polypeptide comprising the amino acid sequence of SEQ ID NO: 1;
(2) a polypeptide comprising an amino acid sequence that has at least 95% sequence identity to the amino acid sequence of SEQ ID NO: 1;
(3) a polypeptide comprising the amino acid sequence of SEQ ID NO: 1, except for an amino substitution selected from the group consisting of Al2E, S80N, R82E, K84E, and combinations thereof in the amino acid sequence of SEQ ID NO: 1; and
(4) a polypeptide comprising the amino acid sequence of SEQ ID NO: 5 or SEQ ID NO: 29.

[claim2]
2. The method according to claim 1, wherein the sample is derived from blood or urine collected from the subject.

[claim3]
3. The method according to claim 1, further comprising:
a testing step of inspecting the presence or absence of a predisposing factor or onset of liver disease or hemolytic disease based on a detection result in the detecting step.

[claim4]
4. The method according to claim 1, wherein the polypeptide is a fusion polypeptide.

[claim5]
5. The method according to claim 1, wherein the polypeptide comprises the amino acid sequence of SEQ ID NO: 1, SEQ ID NO: 5, SEQ ID NO: 29, or SEQ ID NO: 1 except for an amino acid substitution selected from the group consisting of Al2E, S80N, R82E K84E and combinations thereof in the amino acid sequence of SEQ ID NO: 1.

[claim6]
6. The method according to claim 1, wherein the subject is a human, or is a nonhuman animal.

[claim7]
7. The method according to claim 1, where the polypeptide comprises the amino acid sequence of SEQ ID NO: 1.

[claim8]
8. The method according to claim 1, where the polypeptide comprises an amino acid sequence that has at least 95% sequence identity to the amino acid sequence of SEQ ID NO: 1.

[claim9]
9. The method according to claim 1, wherein the polypeptide comprises the amino acid sequence of SEQ ID NO: 1, except for an amino acid substitution selected from the group consisting of Al2E, S80N, R82E, K84E, and combinations thereof in the amino acid sequence of SEQ ID NO: 1.

[claim10]
10. The method according to claim 1, wherein the polypeptide comprises the amino acid sequence of SEQ ID NO: 5 or SEQ ID NO: 29.
  • 発明者/出願人(英語)
  • MIYAWAKI Atsushi
  • KUMAGAI Akiko
  • RIKEN
国際特許分類(IPC)

PAGE TOP

close
close
close
close
close
close