TOP > 外国特許検索 > Human erythroid progenitor cell line comprising HPV E6/E7 operably linked to an inducible promoter and method for producing human enucleated red blood cells

Human erythroid progenitor cell line comprising HPV E6/E7 operably linked to an inducible promoter and method for producing human enucleated red blood cells NEW 実績あり

外国特許コード F190009914
整理番号 08054-US
掲載日 2019年8月26日
出願国 アメリカ合衆国
出願番号 201313745178
公報番号 20140024118
公報番号 8975072
出願日 平成25年1月18日(2013.1.18)
公報発行日 平成26年1月23日(2014.1.23)
公報発行日 平成27年3月10日(2015.3.10)
優先権データ
  • 特願2012-161878 (2012.7.20) JP
発明の名称 (英語) Human erythroid progenitor cell line comprising HPV E6/E7 operably linked to an inducible promoter and method for producing human enucleated red blood cells NEW 実績あり
発明の概要(英語) Provided are: a method for producing an immortalized human erythroid progenitor cell line, enabling efficient and stable production of enucleated red blood cells; and a method for producing human enucleated red blood cells from a human erythroid progenitor cell line obtained by the aforementioned production method. An expression cassette capable of inducing expression of HPV-E6/E7 genes in the presence of DOX was introduced into the genomic DNA of blood stem cells. Then, the blood stem cells were cultured in the presence of DOX and a blood growth factor. Thereby, immortalized cell lines of human erythroid progenitor cells were established. Further, it was revealed that culturing the cell lines under a condition where the expression of the HPV-E6/E7 genes was not induced enabled differentiation induction into enucleated red blood cells at a high ratio.
  • 発明者/出願人(英語)
  • Nakamura; Yukio
  • Kurita; Ryo
  • RIKEN
国際特許分類(IPC)

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