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Human erythroid progenitor cell line comprising HPV E6/E7 operably linked to an inducible promoter and method for producing human enucleated red blood cells NEW_EN achieved

Foreign code F190009914
File No. 08054-US
Posted date Aug 26, 2019
Country United States of America
Application number 201313745178
Gazette No. 20140024118
Gazette No. 8975072
Date of filing Jan 18, 2013
Gazette Date Jan 23, 2014
Gazette Date Mar 10, 2015
Priority data
  • P2012-161878 (Jul 20, 2012) JP
Title Human erythroid progenitor cell line comprising HPV E6/E7 operably linked to an inducible promoter and method for producing human enucleated red blood cells NEW_EN achieved
Abstract Provided are: a method for producing an immortalized human erythroid progenitor cell line, enabling efficient and stable production of enucleated red blood cells; and a method for producing human enucleated red blood cells from a human erythroid progenitor cell line obtained by the aforementioned production method. An expression cassette capable of inducing expression of HPV-E6/E7 genes in the presence of DOX was introduced into the genomic DNA of blood stem cells. Then, the blood stem cells were cultured in the presence of DOX and a blood growth factor. Thereby, immortalized cell lines of human erythroid progenitor cells were established. Further, it was revealed that culturing the cell lines under a condition where the expression of the HPV-E6/E7 genes was not induced enabled differentiation induction into enucleated red blood cells at a high ratio.
  • Inventor, and Inventor/Applicant
  • Nakamura; Yukio
  • Kurita; Ryo
  • RIKEN
IPC(International Patent Classification)

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