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Drive control method for objective lens and fluorescence microscope system UPDATE_EN

Foreign code F190009918
File No. 08183-US
Posted date Aug 26, 2019
Country United States of America
Application number 201414915210
Gazette No. 20160252715
Gazette No. 9921398
Date of filing Aug 25, 2014
Gazette Date Sep 1, 2016
Gazette Date Mar 20, 2018
International application number JP2014004346
International publication number WO2015029408
Date of international filing Aug 25, 2014
Date of international publication Mar 5, 2015
Priority data
  • P2013-175477 (Aug 27, 2013) JP
  • 2014JP04346 (Aug 25, 2014) WO
Title Drive control method for objective lens and fluorescence microscope system UPDATE_EN
Abstract Provided is a driving control method of an objective lens in which an optical-axis chromatic aberration can be corrected by driving the objective lens, and an image can be quickly captured by realizing a quick driving and stabilizing of the objective lens so as to acquire a three-dimensional image at a high speed. A driving control method of the objective lens driven by a piezoelectric actuator provided in a fluorescence microscope includes a first step of applying a pulse voltage larger than a displacement voltage making the objective lens move to a focal position of an observation target to the piezoelectric actuator for a predetermined time so as to move the objective lens near the focal position, and a second step of applying the displacement voltage to the piezoelectric actuator after the first step to stabilize the objective lens.
Outline of related art and contending technology BACKGROUND ART
The fluorescence microscope is a microscope for observing a fluorescent light emitted from a sample of an observation target, and used in various fields such as biology and medicine.
In a case where the observation target is a protein in a cell, in order for the protein to be observed using the fluorescence microscope, the protein is marked by a fluorescent protein (hereinafter, referred to as “marker”) such as Green Fluorescent Protein (GFP), Red Fluorescent Protein (RFP), or Blue Fluorescent Protein (BFP), which is added to the protein of target using a gene engineering method or an immunological method (for example, see Patent Literature 1).
Then, an excitation light is shed at a wavelength appropriate to the type of the fluorescent protein, and the fluorescent light emitted from the fluorescent protein is observed and imaged, so that the sample is observed through the fluorescent protein. As a light source of the excitation light, a laser light source (Ar, Ar―Kr, He―Ne, He―Cd, semiconductor lasers, etc.), an ultrahigh pressure mercury lamp, a xenon lamp, or an ultraviolet LED is used.
Scope of claims [claim1]
1. A driving control method of an objective lens, provided in a fluorescence microscope, to correct an optical-axis chromatic aberration caused by the objective lens which is driven by a piezoelectric actuator, the method comprising:
a first step of applying a pulse voltage to the piezoelectric actuator for a predetermined time so as to move the objective lens near a focal position of an observation target, the pulse voltage being larger than a displacement voltage needed to move the objective lens to the focal position; and
a second step of applying the displacement voltage to the piezoelectric actuator after the first step to stabilize the objective lens.

[claim2]
2. The driving control method of the objective lens according to claim 1, wherein a multiplied voltage of the displacement voltage is applied as the pulse voltage in the first step.

[claim3]
3. A fluorescence microscope system comprising:
a light source;
an objective lens that introduces a light beam emitted from the light source to an observation target;
a piezoelectric actuator that drives the objective lens in an optical axis direction; and
a driving unit that first applies a pulse voltage to the piezoelectric actuator for a predetermined time to move the objective lens near a focal position of the observation target, the pulse voltage being larger than a displacement voltage needed to move the objective lens to the focal position, and then applies the displacement voltage to the piezoelectric actuator so as to stabilize the objective lens.

[claim4]
4. The fluorescence microscope system according to claim 3, wherein the driving unit applies a multiplied voltage of the displacement voltage as the pulse voltage.
  • Inventor, and Inventor/Applicant
  • NAKANO Akihiko
  • ICHIHARA Akira
  • RIKEN
IPC(International Patent Classification)

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