TOP > 外国特許検索 > CELL LABELING AGENT AND CELL LABELING KIT

CELL LABELING AGENT AND CELL LABELING KIT NEW

外国特許コード F190009967
整理番号 (S2018-0349-N0)
掲載日 2019年10月25日
出願国 世界知的所有権機関(WIPO)
国際出願番号 2019JP002440
国際公開番号 WO 2019151128
国際出願日 平成31年1月25日(2019.1.25)
国際公開日 令和元年8月8日(2019.8.8)
優先権データ
  • 特願2018-018172 (2018.2.5) JP
発明の名称 (英語) CELL LABELING AGENT AND CELL LABELING KIT NEW
発明の概要(英語) A cell labeling agent according to the present invention includes a monosaccharide derivative having a six-membered ring structure metabolized by sialic acid in a sialic acid biosynthesis pathway of a cell. Among groups bonded to carbon atoms constituting the six-membered ring in the monosaccharide derivative, at least one group which does not change even when metabolized by the sialic acid biosynthesis pathway includes a ring structure having a carbon-carbon double bond or triple bond.
従来技術、競合技術の概要(英語) BACKGROUND ART
Sialic acid biosynthetic pathway of the monosaccharides by utilizing glucose metabolism, cell surface labeled sugar chains known metabolic labeling method. Metabolic labeling method, for example, an azido group having N- acetyl mannosamine (ManNAc) derivatives (peracetylation-N - azide acetylmannosamine, Ac4ManNAz) are used. Is captured within the cell Ac4ManNAz, deacetylated by the enzyme in the cytoplasm, corresponding to the metabolized azide-acetyl sialic acid N- (SiaNAz). Sugar Sialo SiaNAz is incorporated into the complex. Then, the cell surface oligosaccharides SiaNAz and presented. SiaNAz having an azido group is, the presence of copper azide-alkyne cycloaddition reaction of click by the reaction of such (CuAAC), such as a reporter material having a fluorescent dye can be added.
Non-Patent Document 1 is, for N- (4-butynoyl) peracetylation mannosamine (Ac4ManNAl) using the metabolic labeling method is disclosed. Ac4ManNAl in the cell corresponding to the metabolized to sialic acid (SiaNAl), is presented on the cell surface SiaNAl. Are terminal alkyne have SiaNAl, and alkynes and azides CuAAC the reaction can be, a reporter material to the surface of the cells can be added to the sugar chain.
  • 出願人(英語)
  • ※2012年7月以前掲載分については米国以外のすべての指定国
  • UNIVERSITY OF MIYAZAKI
  • 発明者(英語)
  • XU Yan
  • ISHIZUKA Takumi
  • ZHAO Pei-Yan
国際特許分類(IPC)
指定国 National States: AE AG AL AM AO AT AU AZ BA BB BG BH BN BR BW BY BZ CA CH CL CN CO CR CU CZ DE DJ DK DM DO DZ EC EE EG ES FI GB GD GE GH GM GT HN HR HU ID IL IN IR IS JO JP KE KG KH KN KP KR KW KZ LA LC LK LR LS LU LY MA MD ME MG MK MN MW MX MY MZ NA NG NI NO NZ OM PA PE PG PH PL PT QA RO RS RU RW SA SC SD SE SG SK SL SM ST SV SY TH TJ TM TN TR TT TZ UA UG US UZ VC VN ZA ZM ZW
ARIPO: BW GH GM KE LR LS MW MZ NA RW SD SL SZ TZ UG ZM ZW
EAPO: AM AZ BY KG KZ RU TJ TM
EPO: AL AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HR HU IE IS IT LT LU LV MC MK MT NL NO PL PT RO RS SE SI SK SM TR
OAPI: BF BJ CF CG CI CM GA GN GQ GW KM ML MR NE SN ST TD TG

PAGE TOP

close
close
close
close
close
close