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CELL LABELING AGENT AND CELL LABELING KIT NEW_EN

Foreign code F190009967
File No. (S2018-0349-N0)
Posted date Oct 25, 2019
Country WIPO
International application number 2019JP002440
International publication number WO 2019151128
Date of international filing Jan 25, 2019
Date of international publication Aug 8, 2019
Priority data
  • P2018-018172 (Feb 5, 2018) JP
Title CELL LABELING AGENT AND CELL LABELING KIT NEW_EN
Abstract A cell labeling agent according to the present invention includes a monosaccharide derivative having a six-membered ring structure metabolized by sialic acid in a sialic acid biosynthesis pathway of a cell. Among groups bonded to carbon atoms constituting the six-membered ring in the monosaccharide derivative, at least one group which does not change even when metabolized by the sialic acid biosynthesis pathway includes a ring structure having a carbon-carbon double bond or triple bond.
Outline of related art and contending technology BACKGROUND ART
Sialic acid biosynthetic pathway of the monosaccharides by utilizing glucose metabolism, cell surface labeled sugar chains known metabolic labeling method. Metabolic labeling method, for example, an azido group having N- acetyl mannosamine (ManNAc) derivatives (peracetylation-N - azide acetylmannosamine, Ac4ManNAz) are used. Is captured within the cell Ac4ManNAz, deacetylated by the enzyme in the cytoplasm, corresponding to the metabolized azide-acetyl sialic acid N- (SiaNAz). Sugar Sialo SiaNAz is incorporated into the complex. Then, the cell surface oligosaccharides SiaNAz and presented. SiaNAz having an azido group is, the presence of copper azide-alkyne cycloaddition reaction of click by the reaction of such (CuAAC), such as a reporter material having a fluorescent dye can be added.
Non-Patent Document 1 is, for N- (4-butynoyl) peracetylation mannosamine (Ac4ManNAl) using the metabolic labeling method is disclosed. Ac4ManNAl in the cell corresponding to the metabolized to sialic acid (SiaNAl), is presented on the cell surface SiaNAl. Are terminal alkyne have SiaNAl, and alkynes and azides CuAAC the reaction can be, a reporter material to the surface of the cells can be added to the sugar chain.
Scope of claims (In Japanese)[請求項1]
 細胞のシアル酸生合成経路でシアル酸に代謝される6員環構造の単糖誘導体を含み、
 前記単糖誘導体における6員環を構成する炭素原子に結合した基のうち、前記シアル酸生合成経路で代謝されても変化しない少なくとも1つの基が、
 炭素と炭素との二重結合又は三重結合を有する環構造を含む、
 細胞標識剤。

[請求項2]
 前記単糖誘導体は、
 前記環構造を含む基をRとして、式(I)
[化1]
(省略)
 で表される、
 請求項1に記載の細胞標識剤。

[請求項3]
 前記単糖誘導体は、
 前記環構造を含む基をRとして、式(II)
[化2]
(省略)
 で表される、
 請求項1に記載の細胞標識剤。

[請求項4]
 前記単糖誘導体は、
 前記環構造を含む基をRとして、式(III)
[化3]
(省略)
 で表される、
 請求項1に記載の細胞標識剤。

[請求項5]
 前記Rは、
 下記(a)~(m)
[化4]
(省略)
 からなる群から選択される、
 請求項2から4のいずれか一項に記載の細胞標識剤。

[請求項6]
 請求項1から5のいずれか一項に記載の細胞標識剤と、
 前記細胞の外に提示された、前記シアル酸生合成経路で代謝されても変化しない前記基と反応して前記シアル酸に結合するレポーター物質と、
 を備える、細胞標識キット。
  • Applicant
  • ※All designated countries except for US in the data before July 2012
  • UNIVERSITY OF MIYAZAKI
  • Inventor
  • XU Yan
  • ISHIZUKA Takumi
  • ZHAO Pei-Yan
IPC(International Patent Classification)
Specified countries National States: AE AG AL AM AO AT AU AZ BA BB BG BH BN BR BW BY BZ CA CH CL CN CO CR CU CZ DE DJ DK DM DO DZ EC EE EG ES FI GB GD GE GH GM GT HN HR HU ID IL IN IR IS JO JP KE KG KH KN KP KR KW KZ LA LC LK LR LS LU LY MA MD ME MG MK MN MW MX MY MZ NA NG NI NO NZ OM PA PE PG PH PL PT QA RO RS RU RW SA SC SD SE SG SK SL SM ST SV SY TH TJ TM TN TR TT TZ UA UG US UZ VC VN ZA ZM ZW
ARIPO: BW GH GM KE LR LS MW MZ NA RW SD SL SZ TZ UG ZM ZW
EAPO: AM AZ BY KG KZ RU TJ TM
EPO: AL AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HR HU IE IS IT LT LU LV MC MK MT NL NO PL PT RO RS SE SI SK SM TR
OAPI: BF BJ CF CG CI CM GA GN GQ GW KM ML MR NE SN ST TD TG

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