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MULTI-INPUT/MULTI-OUTPUT GENE SWITCH, AND METHOD FOR PRODUCING SAME NEW 新技術説明会

外国特許コード F190009976
整理番号 (P17-072,S2018-0484-N0)
掲載日 2019年10月28日
出願国 世界知的所有権機関(WIPO)
国際出願番号 2019JP012285
国際公開番号 WO 2019182156
国際出願日 平成31年3月23日(2019.3.23)
国際公開日 令和元年9月26日(2019.9.26)
優先権データ
  • 特願2018-057314 (2018.3.23) JP
発明の名称 (英語) MULTI-INPUT/MULTI-OUTPUT GENE SWITCH, AND METHOD FOR PRODUCING SAME NEW 新技術説明会
発明の概要(英語) [Problem] To provide a method for producing a multi-input/multi-output gene switch or a transcription factor, and a multi-input/multi-output gene switch or transcription factor.
[Solution] A method for producing a multi-input/multi-output gene switch or a transcription factor, said method essentially comprising a step for “fusing at least two transcription factor genes” and for “introducting a mutation into said fused transcription factor gene”, and a multi-input/multi-output gene switch or a transcription factor obtained by using said method.
従来技術、競合技術の概要(英語) BACKGROUND ART
(The current of the biosensor) metabolite or an environment such as monitoring substances, any substance as long as the intracellular concentration of the test in real time, greatly contributes to the development of life sciences. However, the performance of the sensor (sensitivity) is adjusted as it is difficult.
(The configuration of the conventional biosensor) at present, the main principle of operation of the biosensor is the following configuration. 'Gene induction type' sensor, the target compound as a direct transfer on/off downstream of the control mechanism, such as a fluorescent protein reporter places. In the natural world in response to a variety of molecules that are present in the transcription factor, for the majority of metabolites, their sensor motif is not known for. Furthermore, the relevant ones are found, a response to the concentration, the ratio S/N, and in view of the output intensity does not have the ability to withstand practical use in most cases. FRET sensor, in accordance with the target molecule binding if there is a large change in structure, fluorescence resonance energy transfer (FRET) can be constructed using a sensor. However, the rational design and a change in the structure results in a problem that it is very difficult.
(Prior art) the inventors of the present invention, 'thymidine kinase, herpes simplex virus thymidine kinase is preferably derived from a gene sequence encoding the gene upstream and operably connected to at least an expression vector comprising a promoter sequence and used as the selector, a selecting circuit of the gene and a gene switch (reference: Patent Document 1) a method' is disclosed. However, the gene selection method of the circuit, the present invention a multi-input, multi-output type switch or the switch on a gene transcription factors have a different manufacturing method.
The inventors of the present invention, ' DNA repair enzyme DNA alkylation (AAG) alkyl adenine glycosidases gene to the output side, that is designed to operate downstream of the selector is used as an expression vector, gene expression vector is introduced into the expression vector circuit in cells under conditions to induce cell death by alkylating DNA gene and operates the switch by recovering the raw cell, selection may be performed in a short period of ON is found. Further, the ON selection method, the present inventors developed a method of selecting the previously OFF, i.e. the output side of the circuit operation of the gene hsvTK designed such that the selector is used as an expression vector OFF by using a combination of the selection method, selection of ON and OFF in a short time any of the selected, one gene or gene can be carried out at approximately 5-30 of a method for selecting the switch circuit. (See: Patent Document 2) ' has been disclosed. However, the gene selection method of the circuit, the present invention a multi-input, multi-output type switch or the switch on a gene transcription factors have a different manufacturing method.
The inventors of the present invention, 'in the cells for use in modification of the genome of the nucleic acid construct, the nucleic acid construct genome for selecting a cell that has been modified as a gene sequence, nuceoside, preferably containing a gene sequence encoding the thymidine kinase, within a cell and a nucleic acid construct for use in modification of, and the nucleic acid construct can be used within a cell and a method for modifying (reference: Patent Document 3) ' has been disclosed. However, the gene selection method of the circuit, the present invention a multi-input, multi-output type switch or the switch on a gene transcription factors have a different manufacturing method.
  • 出願人(英語)
  • ※2012年7月以前掲載分については米国以外のすべての指定国
  • NATIONAL UNIVERSITY CORPORATION CHIBA UNIVERSITY
  • 発明者(英語)
  • UMENO Daisuke
  • KIMURA Yuki
  • OUCHI Kyohei
  • KAWAI Shigeko
国際特許分類(IPC)
指定国 National States: AE AG AL AM AO AT AU AZ BA BB BG BH BN BR BW BY BZ CA CH CL CN CO CR CU CZ DE DJ DK DM DO DZ EC EE EG ES FI GB GD GE GH GM GT HN HR HU ID IL IN IR IS JO JP KE KG KH KN KP KR KW KZ LA LC LK LR LS LU LY MA MD ME MG MK MN MW MX MY MZ NA NG NI NO NZ OM PA PE PG PH PL PT QA RO RS RU RW SA SC SD SE SG SK SL SM ST SV SY TH TJ TM TN TR TT TZ UA UG US UZ VC VN ZA ZM ZW
ARIPO: BW GH GM KE LR LS MW MZ NA RW SD SL SZ TZ UG ZM ZW
EAPO: AM AZ BY KG KZ RU TJ TM
EPO: AL AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HR HU IE IS IT LT LU LV MC MK MT NL NO PL PT RO RS SE SI SK SM TR
OAPI: BF BJ CF CG CI CM GA GN GQ GW KM ML MR NE SN ST TD TG

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