DIFFERENTIATING AND INDUCING METHOD OF SKELETAL MUSCLE CELL
| Patent code | P09A014414 |
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| File No. | 613 |
| Posted date | May 8, 2009 |
| Application number | P2004-372656 |
| Publication number | P2006-174784A |
| Patent number | P4581085 |
| Date of filing | Dec 24, 2004 |
| Date of publication of application | Jul 6, 2006 |
| Date of registration | Sep 10, 2010 |
| Inventor |
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| Applicant |
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| Title | DIFFERENTIATING AND INDUCING METHOD OF SKELETAL MUSCLE CELL |
| Abstract |
PROBLEM TO BE SOLVED: To provide a method for efficiently and simply differentiating and inducing a bone marrow stromal cell to a skeletal muscle cell with good reproducibility. SOLUTION: This method for differentiating and inducing the bone marrow stromal cell to the skeletal muscle cell includes a process (a) for adding one or more substances selected from a group consisting of a cyclic AMP rise acting medicament, a cyclic AMP analog, and a cell differentiation and survival acting factor to a culture of the bone marrow stromal cell (provided that, the bone marrow stromal cell comprises the cell which is not treated with a demethylating agent), so as to culture the cell, a process (b) for introducing a Notch gene and/or a Notch signaling-associated gene into the cell obtained by the process (a) and culturing the cell, so as to obtain a culture of a myoblast (provided that, the culture does not contain a coculture of the cell into which the gene is introduced and the cell into which the gene is not introduced), and a process (c) for adding a Notch ligand to the culture of the myoblast obtained by the process (b) and culturing the myoblast. |
| Outline of related art and contending technology |
(In Japanese)
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| Field of industrial application |
(In Japanese)
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| Scope of claims |
(In Japanese) 【請求項1】 骨髄間質細胞から骨格筋細胞を分化誘導する方法であって、以下の工程： (a)骨髄間質細胞（ただし、該骨髄間質細胞は脱メチル化剤で処理されていない細胞である）の培養物に、フォルスコリン、並びに細胞分化生存作用性因子である塩基性線維芽細胞成長因子、血小板由来成長因子、及びニューレグリンを添加して培養する工程； (b)上記工程(a)で得られた細胞にNotch細胞内ドメインの遺伝子を導入して培養し筋芽細胞培養物を得る工程（ただし、上記培養は、遺伝子導入された細胞と未導入細胞との共培養を含まない培養である）；及び、 (c)上記工程(b)で得られた筋芽細胞培養物にJagged1蛋白を添加して培養する工程 を含む方法。 【請求項2】 上記工程(c)を無血清培地で行なう請求項1に記載の方法。 【請求項3】 骨髄間質細胞から筋芽細胞を分化誘導する方法であって、以下の工程： (a)骨髄間質細胞（ただし、該骨髄間質細胞は脱メチル化剤で処理されていない細胞である）の培養物に、フォルスコリン、並びに細胞分化生存作用性因子である塩基性線維芽細胞成長因子、血小板由来成長因子、及びニューレグリンを添加して培養する工程；及び (b)上記工程(a)で得られた細胞にNotch細胞内ドメインの遺伝子を導入して培養し筋芽細胞培養物を得る工程（ただし、上記培養は、遺伝子導入された細胞と未導入細胞との共培養を含まない培養である） を含む方法。 |
| IPC(International Patent Classification) |
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| F-term |
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| State of application right | Registered |
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Contact Information for " DIFFERENTIATING AND INDUCING METHOD OF SKELETAL MUSCLE CELL"
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- URL: http://www.saci.kyoto-u.ac.jp/
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