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INTRACELLULAR FAT GLOBULE IMAGING METHOD, IMAGING FLUORESCENT AGENT, AND METHOD FOR MANUFACTURING IMAGING FLUORESCENT AGENT

Patent code	P120006425
File No.	22‐2
Posted date	Jan 10, 2012
Application number	P2010-126562
Publication number	P2011-252786A
Patent number	P5493149
Date of filing	Jun 2, 2010
Date of publication of application	Dec 15, 2011
Date of registration	Mar 14, 2014
Inventor	(In Japanese)秋吉英雄 (In Japanese)藤田恭久
Applicant	(In Japanese)国立大学法人島根大学
Title	INTRACELLULAR FAT GLOBULE IMAGING METHOD, IMAGING FLUORESCENT AGENT, AND METHOD FOR MANUFACTURING IMAGING FLUORESCENT AGENT
Abstract	PROBLEM TO BE SOLVED: To provide a fat globule imaging technology which can be supplied in low cost with short coloring time at high safety. SOLUTION: There is provided a method for manufacturing the imaging fluorescent agent for intracellular fat globules, which is characterized in that the imaging agent is obtained by dispersing fluorescent emissive zinc oxide particles in an isopropyl alcohol solution. In particular, when the concentration of aqueous isopropyl alcohol solution is 60-70%, fluorescent imaging becomes possible suitably. The present invention can be applied not only to biomedical research but also to a food processing field where a use in observing lipid distribution areas is possible for food quality management.
Outline of related art and contending technology	(In Japanese) 近年、生活習慣由来の動脈硬化症や肥満（メタボリックシンドローム）に関して脂肪細胞の基礎的な研究が進められつつある。ここで、細胞内の脂肪球（脂肪滴）の染色には、色素染色法であるオイルレッドO染色、ズダン染色法などが知られている。また、脂肪球の染色に加えて、細胞核やアクチン等を同時に観察することが求められる場合があり、これを実現するために、蛍光抗体法を取り入れた蛍光イメージング技術が有効とされている。現在のところ、蛍光イメージングを実現する脂肪球染色キットとしては、プライマリーセル社製による製品が唯一知られており、これによれば、脂肪球部分が蛍光波長503nmで発光して観察可能となる。しかしながら、従来の技術では以下の問題点があった。従来の脂肪球蛍光イメージング技術は、蛍光剤を反応させるために要する時間が30分程度かかり、迅速な染色ないし蛍光観察ができないという問題点があった。また、安全性については、眼鏡や手袋の保護具が必要であり、人体への接触を避ける必要があり操作性に劣るという問題点があった。また、価格も高額であるという問題点があった。
Field of industrial application	(In Japanese) 本発明は、酸化亜鉛系蛍光技術に関し、特に、細胞内脂肪球のイメージング方法、イメージング用蛍光剤およびイメージング用蛍光剤製造方法に関する。
Scope of claims	(In Japanese) 【請求項1】蛍光発光する酸化亜鉛粒子を濃度60％～70％のイソプロピルアルコール水溶液に分散させて得ることを特徴とする、細胞内脂肪球のイメージング用蛍光剤製造方法。【請求項2】酸化亜鉛粒子の粒径が1nm～5μmであることを特徴とする請求項1に記載の細胞内脂肪球のイメージング用蛍光剤製造方法。【請求項3】蛍光発光する酸化亜鉛粒子を濃度60％～70％のイソプロピルアルコール水溶液に分散させた液を用いて細胞内脂肪球をイメージングすることを特徴とする細胞内脂肪球イメージング方法。【請求項4】酸化亜鉛粒子の粒径が1nm～5μmであることを特徴とする請求項3に記載の細胞内脂肪球イメージング方法。【請求項5】蛍光発光する酸化亜鉛粒子を濃度60％～70％のイソプロピルアルコール水溶液に分散させたことを特徴とする細胞内脂肪球のイメージング用蛍光剤。【請求項6】酸化亜鉛粒子の粒径が1nm～5μmであることを特徴とする請求項5に記載の細胞内脂肪球のイメージング用蛍光剤。
IPC(International Patent Classification)	G01N 21/78 producing a change of colour G01N 21/64 Fluorescence; Phosphorescence C09K 11/54 containing zinc or cadmium C09K 11/08 containing inorganic luminescent materials G01N 33/48 Biological material, e.g. blood, urine; Haemocytometers
F-term	2G043AA03 Observing, examining conditions (e.g., surface conditions) 2G043BA15 Oils, fats 2G043BA16 Biologically-associated substances 2G043DA01 Preparation, pre-treatment of samples 2G043EA01 Fluorescence, photoluminescence 2G043FA01 Variants that measure, scan or photograph the distribution of spaces 2G043FA02 Measurement of microscopically small regions 2G043JA02 (Color) filters 2G043KA02 Visible light 2G043KA03 Ultraviolet light 2G043KA05 Variants where specific wavelength values are mentioned 2G043LA04 Photographic film 2G045AA24 Cell analysis and cell diagnosis 2G045AA25 Examination for illness 2G045BB21 Painting or coating 2G045BB22 Sealing, embedding or fixing 2G045BB24 Staining 2G045BB25 Variants characterized by staining agents 2G045BB42 Variants characterized by diluents 2G045CB01 Cell or tissues 2G045CB17 Animals 2G045CB26 Organs 2G045DA60 Lipids 2G045FA16 Variants using microscopes 2G045FA20 Variants using photographic film 2G045FA26 Variants using visible light 2G045FA27 Variants using ultraviolet rays 2G045FB12 Variants using fluorescent methods 2G045FB16 Variants using slides for analysis 2G045GB01 Entire cells or blood cells 2G045GB03 Cytoplasm 2G045GC12 Degree of coloration 2G045GC15 Amount of luminescence or amount of fluorescence 2G054AA08 Tissues or cells 2G054AB05 Diagnosing or examining illness 2G054CA21 Substances associated with living organisms 2G054CE02 Fluorescent reagents or coloring reagents 2G054EA03 Fluorescent analysis or phosphorescent analysis 2G054EB01 Variants that measure light using a specific wavelength 2G054FA19 Filters or polarizing elements 2G054GA02 Ultraviolet light 2G054GA03 Visible light 2G054GA04 Fluorescence, phosphorescence or excitation light 2G054GA05 Laser light 4H001XA08 Oxygen (O) 4H001XA30 Zinc (Zn)
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State of application right	Registered

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Contact Information for " INTRACELLULAR FAT GLOBULE IMAGING METHOD, IMAGING FLUORESCENT AGENT, AND METHOD FOR MANUFACTURING IMAGING FLUORESCENT AGENT"

Shimane University Department of Intellectual Property, Collaboration Center
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E-mail:
Address: 2, Hokuryou-cho, Matsue-shi, Shimane, Japan , 690-0816
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Contact Information for " INTRACELLULAR FAT GLOBULE IMAGING METHOD, IMAGING FLUORESCENT AGENT, AND METHOD FOR MANUFACTURING IMAGING FLUORESCENT AGENT "

Shimane University Department of Intellectual Property, Collaboration Center
URL: http://www.crc.shimane-u.ac.jp/dip/home.htm
E-mail:
Address: 2, Hokuryou-cho, Matsue-shi, Shimane, Japan , 690-0816
Fax: 81-852-60-2395

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Technology seeds	・ INTRACELLULAR FAT GLOBULE IMAGING METHOD, IMAGING FLUORESCENT AGENT, AND METHOD FOR MANUFACTURING IMAGING FLUORESCENT AGENT

Specification	P5493149
Publication of unexamined applications	P2011-252786A(PDF,536KB)
Patent Gazette	P5493149(PDF,538KB)

Applicant	Shimane University (67) < School organization (7426)
IPC	G01 (1983) C09 (284)
F-term	2G043 (118) 2G045 (351) 2G054 (91) 4H001 (58)

INTRACELLULAR FAT GLOBULE IMAGING METHOD, IMAGING FLUORESCENT AGENT, AND METHOD FOR MANUFACTURING IMAGING FLUORESCENT AGENT

Contact Information for " INTRACELLULAR FAT GLOBULE IMAGING METHOD, IMAGING FLUORESCENT AGENT, AND METHOD FOR MANUFACTURING IMAGING FLUORESCENT AGENT"

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