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(In Japanese)mRNAを運ぶしくみ:制御ネットワークと核の動的機能構造

Research report code R013000016
Posted date Oct 1, 2003
  • (In Japanese)谷 時雄
  • (In Japanese)九州大学大学院理学研究院生物科学部門情報生物学
Research organization
  • (In Japanese)九州大学大学院理学研究院生物科学部門情報生物学
Report name (In Japanese)mRNAを運ぶしくみ:制御ネットワークと核の動的機能構造
Technology summary (In Japanese)mRNAの核から細胞質への輸送は,真核生物の遺伝子発現において欠くことのできない必須過程の一つである。mRNA核外輸送の分子機構を解明するため,単細胞の真核生物である分裂酵母Schizosaccharomyces pombeのmRNA核外輸送温度感受性変異株を分離し(図1),それらの原因遺伝子のクローニングと機能解析を行った。今回の解析によって,mRNA核外輸送だけでなく細胞内の多様な反応に関与する新規なATP/GTP結合タンパク質Ptr7pが見いだされ(図2),また遺伝子の転写及びプロセシング機構とmRNA核外輸送機構との間に密接な関連性があることが明かとなった(図3)。

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Research field
  • Biochemical analytical methods
  • Microscopic methods of living organisms
  • Molecular genetics in general
  • Structure and chemistry of genes
  • Gene expression
Published papers related (In Japanese)(1)Imamura, O., Saeki, K., Tani, T., Ohshima, Y., Sugawara, M., and Furuichi, Y. (1998). Cloning and characterization of a human DEAH-box RNA helicase, a functional homolog of fission yeast Cdc28/Prp8. Nucleic Acids Res. 26, 2063-2068.
(2)Habara, Y., Urushiyama, Y., Tani, T., and Ohshima, Y. (1998). The fission yeast prp10+ gene involved in pre-mRNA splicing encodes a homologue of highly conserved splicing factor, SAP155. Nucleic Acids Res. 26, 5662-5669.
(3)Kanai, N., Yanai. F., Hirose, S., Nibu, K., Izuhara, K., Tani, T., Kubota, T., and Mitsudome, A. (1999). A “G” to “A” transition at the last nucleotide of exon 6 of the gc gene (868G-A) may result in either a splice or missense mutation in patients with X-linked severe combined immunodeficiency. Human Genet. 104, 36-42.
(4)Shibuya, T., Tsuneyoshi, S., Azad, A.K., Urushiyama, S., Ohshima, Y., and Tani, T. (1999). Characterization of the ptr6+ gene in fission yeast: A possible involvement of a transcriptional coactivator TAF in nucleocytoplasmic transport of mRNA. Genetics 152, 869-880.
(5)Beales, M., Flay, N., Mckinney, R., Habara, Y., Ohshima, Y., Tani, T., and Potashkin, J. (2000). Mutations in the large subunit of U2AF disrupt pre-mRNA splicing, cell cycle progression and nuclear structure. Yeast 16, 1001-1013.
(6)Schmidt, W., Richert, K., Gros, T., Habara, Y., Tani, T., and Kaufer, N.F. Prp4p kinase of fission yeast interacts genetically and biochemically with bona fide non-SR splicing factors. EMBO Rep., 2,35-41(2001)
(7)Tatebayashi, T., Tani, T., and Ikeda, H. Fission yeast Toilp, a putative interactor of the cohesin Rad21p, interacts with the small GTPase Ran and is required for mitosis-to-interphase transition, nuclear protein import, and poly(A)+ RNA metabolism. Genetics, 157, 1513-1522 (2001)
(8)Shoubuike, T., Tatebayashi, K., Tani, T., Sugano, S., and Ikeda, H. The dhp1+ gene encoding a putative nuclear 5′-3′ exoribonuclease is required for proper chromosome segregation in fission yeast. Nucleic Acids Res, 29, 1326-1333 (2001)
(9)Habara, Y., Urushiyama, S., Ohshima, Y., and Tani, T. Mutation in the prp12+ gene encoding a homologue of human SAP130/SF3b130 causes differential inhibition of pre-mRNA splicing and arrest of cell cycle progression in Schizosaccharomyces pombe.RNA, 7, 671-681 (2001)
Research project
  • Precursory Research for Embryonic Science and Technology.;Unit Process and Combined Circuit
Information research report
  • (In Japanese)谷 時雄. mRNAを運ぶしくみ 制御ネットワークと核の動的機能構造. 「さきがけ研究21」「素過程と連携」領域 講演要旨集(研究期間1997-2000),2000. p.13 - 25.