Top > Search Research paper > (In Japanese)核酸・多糖複合体における分子認識メカニズムの研究と遺伝子マニュピレーターの創製

(In Japanese)核酸・多糖複合体における分子認識メカニズムの研究と遺伝子マニュピレーターの創製

Research report code R040000052
Posted date May 18, 2007
  • (In Japanese)櫻井 和朗
  • (In Japanese)北九州市立大学国際環境工学部環境化学プロセス工学科
Research organization
  • (In Japanese)北九州市立大学
Report name (In Japanese)核酸・多糖複合体における分子認識メカニズムの研究と遺伝子マニュピレーターの創製
Technology summary (In Japanese)我々が始めて発見した核酸と天然多糖β1-3グルカン複合体に関して、その基礎的性質と構造を明らかにして、複合体形成の機構を探ると共に、機能的オリゴ核酸のデリバリーシステムへの応用を目指した。
Research field
  • Polysaccharides
  • Nucleic acids in general
  • Structure and chemistry of genes
Published papers related (In Japanese)(1) M. Mizu, K. Koumoto, T. Kimura, K. Sakurai, S. Shinkai, Polym. J., 35, 714-720 (2003) “Polysaccharide-polynucleotide complexes Part 17. Solvent effects on conformational-transition of polydeoxyadenylic acid in the complex with schizophyllan”
(2) A. H. Bae, S. W. Lee, M. Ikeda, M. Sano, S. Shinkai, K. Sakurai, Carbohydr. Res., in press. “Rod-like architecture and helicity of the poly(C)/schizophyllan complex as observed by AFM and SEM”
(3) M. Mizu, K. Koumoto, T. Kimura, K. Sakurai, S. Shinkai, Biomaterials, in press. “Protection of polynucleotides against nuclease-mediated hydrolysis by complexation with schizophyllan”
(4) K. Sakurai, R. Iguchi, M. Mizu, K. koumoto, S. Shinkai, Bioorg. Chem., 31, 216-226 (2003). “Polysaccharide-Polynucleotide Complexes Part 7. Hydrogen-ion and Salt Concentration Dependence of Complexation between Schizophyllan and Single-Stranded Homo RNAs”
(5) K. Sakurai, R. Iguchi, K. Koumoto, T. Kimura, M. Mizu, Y. Hisaeda, S. Shinkai, Biopolymers, 65, 1-9 (2002). “Polysaccharide-polynucleotide Complexes Part 8. Cation Induced Complex Fromation between Polyuridylic Achid and Schizophyllan”
(6) K. Sakurai, M. Mizu, S. Shinkai, Biomacromolecules, 2, 641-650 (2001). “Complementary polynucleotide minmic behavior of a Natural polysaccharide: Schizophyllan in the Macromolecular Complex with a Single stranded RNA: Poly(C)”
(7) M. Mizu, T. Kimura, K. Koumoto, K. Sakurai, S. Shinkai, Chem. Commun., 2001, 429-430. “Thermally induced conformational-transition of polydeoxyadenosine in the complex with schizophyllan and the base-1ength dependence of its stability”
(8) K. Koumoto, T. Kimura, M. Mizu. K. Sakurai, S. Shinkai, Chem. Commun., 2001, 1962-1963. “Chemical modification of schizophyllan by introduction of a cationic charge into the side chain which enhances the thermal stability of schizophyllan / poly(C) complexes”
(9) K. Sakurai, S. Shinkai, J. Am. Chem. Soc., 122, 4520-4521 (2000). “Molecular recognition of Adenine, Cytosine, and Uracil in a Single-Stranded RNA by a Natural polysaccharide: Schizophyllan”
(10) 櫻井和朗,新海征治 多糖・核酸からなる3重らせんの発見とその応用高分子,51 (8), 603-606 (2002)
Research project
  • Precursory Research for Embryonic Science and Technology.;Organization and Function
Information research report
  • (In Japanese)櫻井 和朗. 核酸・多糖複合体における分子認識メカニズムと遺伝子工学への応用. 「さきがけ研究」「組織化と機能」領域 研究報告会講演要旨集(研究機関2000-2003),2003. p.18 - 19.