Top > Search Research paper > (In Japanese)腸管寄生原虫赤痢アメーバの病原機構の解明

(In Japanese)腸管寄生原虫赤痢アメーバの病原機構の解明

Research report code R070000067
File No. R070000067
Posted date Apr 11, 2008
  • (In Japanese)野崎 智義
  • (In Japanese)国立感染症研究所寄生動物部
Research organization
  • (In Japanese)国立感染症研究所寄生動物部
Report name (In Japanese)腸管寄生原虫赤痢アメーバの病原機構の解明
Technology summary (In Japanese)嫌気性原虫赤痢アメーバはヒトの大腸に寄生し、複数の病原因子を細胞外に放出して免疫細胞を融解したり、感染局所の組織破壊を起こすとともに、破壊された細胞・組織を活発に貧食(ファゴサイトーシス)・除去して寄生を成立させる。病原因子の分泌や貧食は赤痢アメーバの寄生に不可欠である。その分子機構を解明することは、赤痢アメーバの病原機構を理解するために不可欠である。我々は分泌や貧食を調節する細胞機構としての小胞輸送に着目し、赤痢アメーバの小胞輸送の特殊性を分子レベルで明らかにすることにより、赤痢アメーバの小胞輸送が病原機構にどう関与するかを解明することを目指した。

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Research field
  • Physiology and pathogenicity of microbial infection
Published papers related (In Japanese)(1) Haghighi, A., Kobayashi, S., Takeuchi, T., Masuda, G., and Nozaki, T.(2002) Remarkble genetic polymorphism among Entamoeba histoltyica isolates from a limited geographic area. J. Clin, Microbiol, 40, 4081-90.
(2) Haghighi, A., Kobayashi, S., Takeuchi, T., Thammapalerd, N., and Nozaki, T.(2003) Geograhic diversity of genotypes among Entamoeba histolytica field isolates. J. Clin, Microbiol., 41, 3748-3756.
(3) Dvorak, J.A., Kobayashi, S., Nozaki, T., Takeuchi, T. and Matsubara, C.(2003) Induction of permeability changes and death of vertebrate cells is modulated by the virulence of Entamoeba spp. Isolates. Parasitol. Int. 52, 169-173.
(4) Ali, V., Shigeta, Y., and Nozaki, T., (2003) Molecular and structural characterzation of NADPH-dependent D-glycerate dehydrogenase from the enteric parasitic protist Entamoeba histolytica. Biochem. J.375, 729-736.
(5) Tokoro, M., Kobayashi, S., Takeuchi, T., and Nozaki, T.(2003) A novel sulfur-containing amino acid degradation enzyme methionine γ-lyase from the enteric protozoan parasite Entamoeba histoltyica. J. Biol. Chem. 278, 42717-42727
(6) Ghosh, S., Chan, J., Lea, C.R., Meints, G.A., Lewis, J.C., Tovian, Z., Flessner, R., Loftus, T.C., Bruchhaus, I., Fradley, K.I., Kendrick, H., Croft, S., Kemp, R., Kobayashi, S., Nozaki, T., and Oldfield, E. (2004) Effects of Bisphosphonates on the Growth of Entamoeba and Plasmodium species in vitro and in vivo. J. Med. Chem. 47, 175-187.
(7) Kumagai, M. Makioka, A., Takeuchi, T., and Nozaki, T. (2004) Molecular cloning and characterization of a protein farnesyltransferase from the enteric protozoan parasite Entamoeba histolytica. J.Biol. Chem. 279, 2316-2323.
(8) Ali, V., Shigeta, Y., Tokumoto, U., Takahashi, Y., and Nozaki, T. (2004) An intestinal parasitic protist Entamoeba histolytica possesses a non-redundant NIF-like system for iron-sulfur cluster assembly under anaerobic conditions. J. Biol. Chem. 279, 16863-16874.
(9) Ali, V., Shigeta, Y., Hashimoto, T., and Nozaki, T. (2004) Molecular and biochemical characterization of D-phosphoglycerate dehydrogenase from Entamoeba histolytica: a unique enteric protozoan parasite that possesses both phosphorylated and non-phosphorylated serine metabolic pathways. Eur. J. Biochem. 271, 2670-2681.
(10) Saito-Nakano, Y., Yasuda, and Nozaki, T. (2004) Unique role of Rab5 and Rab7 in phagocytosis of the enteric protozoan parasite Entamoeba histolytica. J. Biol. Chem. 279, 49497-49507.
Research project
  • Precursory Research for Embryonic Science and Technology.;Host and Defense
Information research report
  • (In Japanese)野崎 智義. 腸管寄生原虫赤痢アメーバの病原機構の解明. 「生体と制御」研究領域 第一回研究報告会感染症と免疫疾患の発病メカニズムの解明へ新しいアプローチ 要旨集, 2004. p.24 - 26.