Top > Search Research paper > (In Japanese)非水系のナノ集合体と生体分子の融合による新機能創製 -非水系でのナノ集合体と生体分子の融合による機能変換と制御-

(In Japanese)非水系のナノ集合体と生体分子の融合による新機能創製 -非水系でのナノ集合体と生体分子の融合による機能変換と制御-

Research report code R070000090
File No. R070000090
Posted date Apr 11, 2008
  • (In Japanese)後藤 雅宏
Report name (In Japanese)非水系のナノ集合体と生体分子の融合による新機能創製 -非水系でのナノ集合体と生体分子の融合による機能変換と制御-
Technology summary (In Japanese)生体分子は、当然のことながら水中で効率よく機能するように作られている。本研究では、このような成体分子に、非水媒体という新たな機能発現の場を与えることがねらいである。通常、生体分子は、非水溶媒中では高次構造を保持できず、その機能を失うことが多い。このため非水系で生体分子機能を引き出すためには、何らかの工夫が必要となる。本研究では、生体分子の機能発現のために非水系で熱力学的に安定に形成されるナノ集合体を利用した。非水系における代表的なナノ集合体を、逆ミセル(Fig.1) と呼ぶが、逆ミセルは、非水系にナノオーダーの安定な微水環境を与えることができる。タンパク質や拡散(DNA)に代表される生体分子は、おおむねナノオーダーの大きさを有しているため、生体分子をその殻の中に安定に保持できる。本さきがけ研究では、このナノ集合体が形成する特異なナノ空間を、積極的に生体分子の機能発現の場として利用した。これらナノ集合体が作り出す特異環境はバルクの水のそれと大きく異なるため、取り込まれた酵素やタンパク質、DNAといった生体分子が、これら制限されたナノ空間で特異な挙動を示すことを期待した。

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Research field
  • Applications of enzymes
Published papers related (In Japanese)(1) Catalytic oxidation of o-phenylendiamine by cytochrome c encapsulated in reversed micelles J. Mol. Catal. B: Enzymatic, 11, 955-959 (2001), T. Ono, K. Kawakami, M. Goto, S. Furusaki
(2) Preparation and Catalytic Performance of Surfactant-Manganese Peroxidase-MnII Ternary Comple in Organic Media, Enzyme and Microbial Technology, 28, 329-332 (2001) S. Okazaki, M. Goto, S. Furusaki, H. Wariishi, H. Tanaka
(3) Chataritic Performance of Lignine Peroxidase Alip-P3 in reversed Micelles Biochemical Engineering J., Vol.8, No. 2, 129-134 (2001) J. Michizoe, S. Okazaki, M.Goto, S. Furusaki
(4) Catalytic Activity of Laccase Hosted in Reversed Micelles J. Bioscience and Bioehg., Vol.92, No.1,67-71 (2001), J. Michizoe, M. Goto, S. Furusaki
(5) Complex Formation of Cytochrome c with a Calixarene Carboxylic Acid Derivative: A Novel Solubilization Method for Biomolecules in Organic Media Biomacromolecules, Vol. 3, No. 3, 438-444 (2002), T. Oshima, M. Goto, S. Furusaki
(6) Remarkably Enhanced Inhibitory Effects of Three-Component Hybrid Liposomes Including Sugar Sufactants on the Growth of Lung Carcinoma Cells Chem. Pharm. Bull., 50(4), 563-565 (2002) R. Ueoka, Y. Matsumoto, S. Hirose, K. Goto, M. Goto, S. Furusaki
(7) Enantioselective Polymer Prepared by Surface Imprinting Techniques Anal. Chimica Acta, Vol. 469. N0.2, 173-181 (2002), K. Araki, M. Goto, S. Furusaki
(8) Polyethylene Glycol-Lipase Complex That is Catalytically Active for Alcoholysis Reactions in Ionic Liquids Biotechnology Letters, 24, 1341-1345 (2002), T. Maruyama, S. Nagasawa, M. Goto
(9) A Molecularly Imprinted Polymer that shows Enzymatic Activity Biochem. Eng. J. 14, 85-91 (2003), T. Eiichi, K. Uezu, M. Goto, S. Furusaki
(10) Oxidation of Bisphenol A Catalysed by Laccase in Reversed Micelles in Organic Media Enzy. Microb. Technol., 31, 227-232(2002) S. Okazaki, J. Michizoe, M. Goto, S. Furusaki, H. Wariishi, H. Tanaka
(11) Enzymatic Synthesis of Sugar Amino Acid Esters in Organic Solvents J. Biosci, Bioeng., Vol. 94, No. 4, 357-361(2002), T. Maruyama, S. Nagasawa, M. Goto
(12) DNA Hybridization in Reverse Micelles and its Application to Mutation Detection Analyst 128(2), 161-165(2003), Lian-Chun Park, T. Maruyama, M. Goto
(13) Hypotlycemic effect of surfactant-coated insulin solubilized in a novel (S/O/W) emulsion Int. Phorm. Chem.Vol. 252, 271-274 (2003), E. Toorisaka, N. Kamiya, H. Ono, M. Goto
(14) Control of water content by reverse micellar solutions for peroxidase catalysis in a water-immiscible organic solvent J. Biosci. Bioeng., Vol. 95, No. 4, 425-427(2003), J. Michizoe, T. Maruyama, M. Goto
(15) Use of ionic liquids in a lipase-facilitated supported liquid membrane. Biotechnol. Lett. 25, 805-808, (2003), E. Miyako, T. Maruyama, N. Kamiya, M. Goto
(16) Discriminate surface molecular recognition sites on a microporous substrate: A new approach Macromolecules, 36 (12): 4472-4477 (2003), Han MN, Kane R, Goto M, Belfort G
(17) Protein Refolding in Nanostructured Reversed Micelles Including a Molecular Chaperone J. Biosci. Bioeng., Vol. 96, No. 3, 275-278(2003), M. Sakono, H. Ichinose, M. Goto
(18) Liquid membrane transport of amino acids by a calyx[6]arene carboxylic acid derivative J. Membrane Sci., Vol. 217, 87-97(2003), T. Oshima, K. Inoue, S. Furusaki, M. Goto
(19) Simple detection of point mutations in DNA oligonucleotides using SYBR GreenI Biotechnol. Lett. 25, 1637-1641 (2003), T. Maruyama, T. Takata, N. Kamiya, M. Goto
(20) Can lipases hydrolyze peptide bond? Enz. Microb. Technol., Vol. 32, 655-657(2003), T. Maruyama, M. Nakajima, M. Seki, M. Goto
(21) Transport of Organic Acids through a Supported Liquid Membrane Driven by Lipase-Catalyzed Reactions J. Biosci. Bioeng., 96(4), 370-374 (2003), E. Miyako, T. Mayuyama, N. Kamiya, M. Goto
(22) Enzymatic degradation of p-chlorophenol in a two-phase flow microchannel system. Lab. on a Chip, 3, 315-319 (2003), T. Maruyama, J. Uchida, T. Ohkawa, M. Goto
(23) Enantioselective transport of (S)-ibuprofen through a lipase-facilitated supported liquid membrane based on ionic liquids. Chem. Commun., 2926-2927(2003) E. Miyako, T. Maruyama, N. Kamiya, M. Goto
(24) Activation of manganese peroxidase in an organic mediurn using a mediator. Biochem Eng J, Vol. 19 (1), 43-46(2004), J. Michizoe, T. Maruyama, N. Kamiya, M. Goto,
(25) DNA hybridization in nanpstructural molecular assemblies enables detection of gene mutations without a fluorescent probe. Biomacromolecules, 5, 49-53 (2004). T. Maruyama, L.C. Park, T. Shinohara, M. Goto,
(26) Poly(ethylene glycol)-lipase complexes catalytically-active in fluorous solvents. Org. Biomol. Chem. 2, 524-527 (2004). T. Maruyama, T. Kotani, N. Kamiya, M. Gdto,
(27) First application of calixarenes as extractants in room-temperature ionic liquids. Chemistry Lett. Vol.33, No. 3, 320-321(2004), K. Shimojo, M. Goto,
(28) Dominant factors affecting extraction behavior of amino compounds by a calyx[6]arene carboxylic acid derivative , Anal. Chim. Act., 509, 137-144(2004) T. Oshima, K. Inoue, K. Uezu, M. Goto
(29) Refolding of denatured carbonic anhydrase B by reversed micelles formulated with nonionic surfactant. Biochem. Eng. J., 19, 217-220 (2004), M. Sakono, N. Kamiya, M. Goto
(30) Poly(ethylene glycol)-lipase complex highly active and enantioselective in ionic liquids. Org. Biomol. Chem., 2(8), 1239-1244 (2004). T. Maruyama, T. Kotani, N. Kamiya, M. Goto
(31) Eleotron-transfer reactions and function of Cyiochrome P450cam monooxygenase system in reverse micelles. Langmuir 20, 5561-5568 (2004) H. Ichinose, J. Michizoe, T. Maruyama, N. Kamiya and M. Goto,
(32) Factors affecting the oxidative activiy of laccase towards biphenyl derivatives in homogeneous aqueous-nonaqueous systems. J. Biosci. Bioeng., Vol. 98, No. 1., 14-19 (2004) J. Tominaga, J. Michizoe, N. Kamiya, H. Ichnose, T. Matuyama, M. Goto
(33) Liquid membrane operations in a microfluidic device for selective separation of metal ions. Anal. Chem. 76, 4495-4500 (2004). T. Maruyama, H. Matsushita, J. Uchida, F. Kubota, N. Kamiya, M. Goto
(34) Solvent extraction ahd stripping of silver Ions in room-temperature ionic liquids. Anal. Chem. 76, 5039-5044 (2004), K. Shimojo, M. Goto
(35) Calix[6]arene acetic acid extraction behavior and specificity with respect to nucleobases. Anal. Chim. Acta., 521, 163-171 (2004), K. Shimojo, T. Oshima, M.Goto
(36) Highly enantioselective separation using a supported liquid membrane encapsulating surfactant-enzyme complex. J. Am. Chem. Soc. 126, 8622-8623 (2004) E. Miyako, T. Maruyama, N. Kamiya, M. Goto
Research project
  • Precursory Research for Embryonic Science and Technology.;Conversion and Control by Advanced Chemistry
Information research report
  • (In Japanese)後藤 雅宏. 非水系のナノ集合体と生体分子の融合による新機能創製 -非水系でのナノ集合体と生体分子の融合による機能変換と制御-. さきがけライブ2004 物質科学・生命分野 さきがけタイプ研究報告会 「変換と制御」領域 講演要旨集(第二期研究者)(研究期間2001-2004), 2004. p.89 - 102.