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(In Japanese)タンパク質オルガネラ移行と遺伝子発現の非侵襲的時空間解析法の確立

Research report code R070000212
File No. R070000212
Posted date Apr 11, 2008
Researchers
  • (In Japanese)小澤 岳昌
Affiliation
  • (In Japanese)自然科学研究機構分子科学研究所
Research organization
  • (In Japanese)自然科学研究機構分子科学研究所
Report name (In Japanese)タンパク質オルガネラ移行と遺伝子発現の非侵襲的時空間解析法の確立
Technology summary (In Japanese)環境変化等の細胞外刺激が引き起こす遺伝子の発現とタンパク質の細胞内諸器官(オルガネラ)への移行は,細胞が効率的かつ協調的に機能するための重要な仕組みである。オルガネラ特有の機能を解明するためには,細胞が生きた状態で,オルガネラに局在する生体分子の機能を解析する新たな技術が必用である。本研究では,二分した蛍光あるいは発光タンパク質を細胞内で再構成させる新たな手法を用いて,生きた細胞内での遺伝子の発現とタンパク質のオルガネラへの移行の詳細を時空間解析する新しい研究手法の確立を目指した。
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Research field
  • Gene expression
  • Cell physiology in general
Published papers related (In Japanese)(1) High-Throughput Sensing and Noninvasive Imaging of Protein Nuclear Transport by Using Reconstitution of Split Renilla Luciferase. S. B. Kim*, T. Ozawa*, S. Watanabe, Y. Umezawa, Proc. Natl. Acad. Sci. USA., 101, 11542-11547 (2004). (* Equal contribution to this work)
(2) Methods of Analysis for Protein Dynamics in Living Cells Based on Protein Splicing. T. Ozawa, Bull Chem. Soc. Jpn., 78, 739-751 (2005).
(3) A High-Throughput Screening of Genes that Encode Proteins Transported into the Endoplamsic Reticulum in Mammalian Cells, T. Ozawa, K. Nishitani, Y. Sako, Y. Umezawa, Nucleic. Acids Res., 33, e34 (2005).
(4) Genetically Encoded Stress Indicator for Noninvasively Imaging Endogenous Corticosterone in Living Mice. S.B. Kim, T. Ozawa, Y. Umezawa, Anal. Chem., 77, 6588-6593 (2005).
(5) A Genetically Encoded Indicator for Assaying Bioactive Chemicals that Induce Nuclear Transport of Glucocorticoid Receptor. S.B. Kim, T. Ozawa, Y. Umezawa, Anal. Biochem., 347, 213-220 (2005).
(6) Quantitative Determination of Protein Nuclear Transport Induced by Phosphorylation or by Proteolysis. S.B. Kim, R. Takao, T. Ozawa, Y. Umezawa, Anal. Chem., 77, 6928-6934 (2005).
(7) Designing Split Reporter Proteins for Analytical Tools. T. Ozawa, Anal. Chim. Acta, 556, 58-68 (2006).
(8) A. Method for Determining the Activities of Cytokines based on the Nuclear Transport of Nuclear Factor-Kappa B. S.B. Kim, Y. Natori, T. Ozawa, H. Tao, Y. Umezawa, Anal. Biochem., in press.
(9) A Genetically Encoded Optical Probe for Detecting Release of Proteins from Mitochondria toward Cytosol in Living Cells and Animals. A. Kanno, T. Ozawa and Y. Umezawa, Anal. Chem., in press.
(10) A Short Peptide Sequence that Targets Fluorescent and Functional Proteins into the Mitochondrial Intermembrane Space. T. Ozawa, Y. Natori, Y. Sako, H. Kuroiwa, T. Kuroiwa and Y. Umezawa, submitted.
(11) Imaging Endogenous RNA in Single Living Cells; Dynamics of Mitochondrial RNA during Oxidative Stress. T. Ozawa, Y. Natori and Y. Umezawa, submitted.
(12) 32nd Annual Meeting of the American Society for Photobiology, Seattle, USA; July (2004).
Research project
  • Precursory Research for Embryonic Science and Technology.;Structure and Function of Biomolecules
Information research report
  • (In Japanese)小澤 岳昌. タンパク質オルガネラ移行と遺伝子発現の非侵襲的時空間解析法の確立. 2006年度“たんぱく質関連領域”合同シンポジウム 要旨集 「生体分子の形と機能」, 2006. p.9 - 12.

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