Top > Search Research paper > (In Japanese)環境低負荷型の社会システム 環境低負荷型の高分子物質生産システムの開発

(In Japanese)環境低負荷型の社会システム 環境低負荷型の高分子物質生産システムの開発

Research report code R990004344
Posted date Feb 6, 2001
  • (In Japanese)土肥 義治
  • (In Japanese)理化学研究所
Research organization
  • (In Japanese)理化学研究所高分子化学研究室
Report name (In Japanese)環境低負荷型の社会システム 環境低負荷型の高分子物質生産システムの開発
Technology summary (In Japanese)生物の物質生産機能を利用して、再生可能な生物有機資源や二酸化炭素から新しい高分子材料を創成する技術開発を行っている。高分子化学、材料科学、環境科学の領域で活躍する研究者の共同により、高分子物質の合成、生産生物育種、効率的生産、高機能材料化、生分解性制御、環境アセスメントなど一連の基礎研究を行った。微生物は多様な炭素資源から酵素作用で高分子を生産可能である。第四の生体高分子として注目を浴びるバイオポリエステルに重点をおいて研究を進めた。その生合成、機能解明、構造解析、遺伝子解析、リサイクル技術などについて検討した。新規ポリエステルを合成する3種の細菌(例えば、Aeromonas caviae)について育種と遺伝子解析、ポリマー合成の機構解明、植物体へのポリエステル生合成遺伝子の導入などを試みた。また、生分解性ポリエステルの材料設計と機能開発、ポリマー加水分解酵素の構造解析と機能解明を行った。
Research field
  • Enzyme physiology
  • Polyesters, alkyd resins
  • Polymer waste treatment
  • Substance production by microorganisms, cultured tissues/cells in general
  • Substance production by plant tissues and cells
Published papers related (In Japanese)(1)T. Fukui, N. Shiomi and Y. Doi: Expression and Characterization of (R)-Specific Enoyl Coenzyme A Hydratase Involved in Polyhydroxyalkanoate Biosynthesis by Aeromonas caviae; J. Bacteriol., 180 667-673(1998)
(2)H. Abe, Y. Doi, H. Aoki and T. Akehata: Solid-State Structures and Enzymatic Degradabilities for Melt-Crystallized Films of Copolymers of (R)-3-Hydroxybutyric Acid with Different Hydroxyalkanoic Acids; Macromolecules, 31 1791-1797(1998)
(3)T. Iwata and Y. Doi: Morphology and Enzymatic Degradation of Poly(L-lactic acid) Single Crystals; Macromolecules, 31 2461-2467(1998)
(4)H. Abe, H. Aoki and Y. Doi: Morphologies and Enzymatic Degradability of Melt-Crystallized Poly(3-hydroxybutyric acid-co-6-hydroxyhexanoic acid); Macromol. Symp., 130 81-89(1998)
(5)T. Fukui and Y. Doi: Efficient Production of Polyhydroxyalkanoates from Plant Oils by Alcaligenes eutrophus and Its Recombinant Strain; Appl. Microbiol. Biotechnol., 49 333-336(1998)
(6)K. Kasuya, K. Takagi, S. Ishiwatari, Y. Yoshida and Y. Doi: Biodegradabilities of Various Aliphatic Polyesters in Natural Waters;Polym. Degrad. Stab., 59 327-332(1998)
(7)A. Cao, K. Kasuya, H. Abe, Y. Doi and Y. Inoue: Studies on Comomer Compostional Distribution of the Bacterial Poly(3-hydroxybutyric acid-co-3-hydroxypropionic acid)s and Thermal Characteristrics of Their Fractionated Component Copolyesters; Polymer, 20 4801-4816(1998)
(8)M. Shinomiya, T. Iwata and Y. Doi: The Adrorption of Substrate-Binding Domain of PHB Depolymerases to the Surface of Poly(3-hydroxybutyric acid); Int. J. Biol. Macromol., 22 129-135(1998)
(9)J. Li, J. Uzawa and Y. Doi: Conformational Analysis of Oligomers of (R)-3-Hydroxybutanoic Acid in Solutions by 1H NMR Spectroscopy; Bull. Chem. Soc. Jpn., 71 1683-1689(1998)
(10)J. Li, J. Uzawa and Y. Doi: NMR Spectroscopic Studies on Complex Formation Between Dimeric (R)-3-Hydroxybutanoic Acid and β-Cyclodextrin; Bull. Chem. Soc. Jpn., 71 1953-1957(1998)
(11)T. Fukui, M. Kato, H. Matsusaki, T. Iwata and Y. Doi: Morphological and 13C-Nuclear Magnetic Resonance Studies for Polyhydroxyalkanoate Biosynthesis in Pseudomonas sp. 61-3; FEMS Microbiol. Lett., 164 219-225(1998)
(12)S. Kusaka, T. Iwata and Y. Doi: Microbial Synthesis and Physical Properties of Ultra-High-Molecular-Weight Poly[(R)-3-hydroxybutyrate]; J. Macromol. Sci., Pure Appl. Chem., A35 319-335(1998)
(13)K. Sudesh, T. Fukui and Y. Doi: Genetic Analysis of Comamonas acidovorans Polyhydroxyalkanoate Synthase and Factors Affecting the Incorporation of 4-Hydroxybutyrate Monomer; Appl. Environ. Microbiol., 64 3437-3443(1998)
(14)H. Matsusaki, S. Manji, K. Taguchi, M. Kato, T. Fukui and Y. Doi: Cloning and Molecular Analysis of the Poly(3-hydroxybutyrate) and Poly(3-hydroxybutyrate-co-3-hydroxyalkanoate) Biosynthesis Genes in Pseudomonas sp. Strain 61-3; J. Bacteriol., 180 6459-6467(1998)
(15)S. Matsumura, Y. Suzuki, K. Tsukada, K. Toshima, Y. Doi and K. Kasuya: Lipase-Catalyzed Ring-Opening Polymerization of β-Butyrolactone to the Cyclic and Linear Poly(3-hydroxybutyrate); Macromolecules, 31 6444-6449(1998)
Research project
  • Core Research for Evolutional Science and Technology;Social Systems for Better Environment Performance
Information research report
  • (In Japanese)土肥 義治. 環境低負荷型の社会システム 環境低負荷型の高分子物質生産システムの開発. 戦略的基礎研究推進事業 平成10年度 研究年報,1999. p.797 - 801.