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(In Japanese)組換えを介したゲノム動態制御

Research report code R000000240
Posted date Sep 30, 2002
  • (In Japanese)柴田 武彦
  • (In Japanese)理化学研究所
Research organization
  • (In Japanese)理化学研究所
Report name (In Japanese)組換えを介したゲノム動態制御
Technology summary (In Japanese)本研究では,ゲノムを変化させる一方,逆に変異を取り除く組替えを介したゲノム動態制御について,遺伝子・分子機能から染色体・細胞の挙動までの総合的な理解と高等動物での普遍性の検証,新技術の基盤構築を目指す。以下の課題について研究する,
Research field
  • Molecular genetics in general
  • Gene manipulation
Published papers related (In Japanese)(1)Furuse, M., Nagase, Y., Tsubouchi, H., Murakami-Murofushi, K., Shibata, T. and Ohta, K. (1998) "Distinct roles of two separable in vitro activities of yeast Mre11 in mitotic and meiotic recombination." EMBO J., 17, 6412-6425.
(2)Ohta, K., Wu, T.-C., Lichten, M. and Shibata, T. (1999) Competitive inactivation of a double-strand DNA break site involves parallel suppression of meiosis-induced changes in chromatin configuration. Nucleic Acids Res., 27, 2175-2180.
(3)Aihara, H., Ito, Y., Kurumizaka, H., Yokoyama, S. and Shibata, T. (1999) "The N-terminal domain of the human Rad51 protein binds DNA: Structure and a DNA binding surface as revealed by NMR." J. Mol. Biol., 290, 495-504.
(4)Kurumizaka, H., Aihara, H., Kagawa, W., Shibata, T. and Yokoyama, S. (1999) "Human Rad51 amino acid residues required for Rad52 binding." J. Mol. Biol., 291, 537-548.
(5)Kurumizaka, H., Ikawa, S., Sarai, A., Shibata, T. (1999) "The mutant RecA proteins, RecAR243Q and RecAK245N, that exhibit defective DNA binding in homologous pairing." Arch. Biochem. Biophys., 365, 83-91.
(6)Rajesh, S., Sakamoto, T., Iwamoto-Sugai, M., Shibata, T., Kohno, T., Ito, Y. (1999) "Ubiquitin binding interface mapping on yeast ubiquitin hydrolase by NMR chemical shift perturbation." Biochemistry, 38, 9242-9253
(7)Senbongi, H., Ling, F., Shibata, T. (1999) "A mutation in a mitochondrial ABC transporter results in mitochondrial dysfunction through oxidative damage of mitochondrial DNA." Mol. Gen. Genet., 262, 436-436
(8)Yamada, T., Okuhara. K., Iwamatsu, A., Seo, H., Ohta, K., Shibata, T. and Murofushi, H. (2000) "p97 ATPase, an ATPase involved in membrane fusion, interacts with DNA unwinding factor (DUF) that functions in DNA replication," FEBS Lett., 466, 287-291.
(9)Jeong, S.-M., Kawasaki, K., Juni, N. and Shibata, T. (2000) "Identification of Drosophila melanogaster RECQE as a member of a new family of RecQ homologues preferentially expressed in early embryos," Mol. Gen. Genet., 263, 183-193.
(10)Kurumizaka, H., Aihara, H., Ikawa, S. and Shibata, T. (2000). "Specific defects in double-stranded DNA unwinding and homologous pairing of a mutant RecA protein," FEBS Lett., 477, 129-134.
(11)Fox, M. E., Yamada, T., Ohta, K. and Smith, G. R. (2000) "A Family of CRE-related DNA sequences with meiotic recombination hotspot activity in Schizosaccharomyces pombe," Genetics, 156, 59-68.
(12)Miyajima, A., Seki, M., Onoda, F., Shiratori, M., Odagiri, N., Ohta, K., Kikuchi, Y., Ohno, Y. and Enomoto, T. (2000) "Sgsl helicase activity is required for mitotic but apparently not for meiotic functions," Mol. Cell. Biol., 20, 6399-6409.
(13)Ling, F., Morioka, H., Ohtsuka. E. and Shibata, T. (2000). "A role for MHR1, a gene required for mitochondrial genetic recombination, in the repair of damage spontaneously introduced in yeast mtDNA," Nucleic Acids Res., 28, 4956-4963.
(14)Smith, K, N., Penkner, A., Ohta, K., Klein, F. and Nicolas, A. (2001) "B-type cyclins CLB5 and CLB6 control the initiation of recombination and synaptonemal complex formation in yeast meiosis," Current Biology 11, 88-97.
(15)Kurumizaka, H., Ikawa, S., Nakada, M., Eda, K., Kagawa, W., Takata, M., Takeda, S., Yokoyama, S. and Shibata, T. (2001) "Homologous-pairig activity of the human DNA-repair proteins Xrcc3 ・ Rad51C." Proc. Natl. Acad. Sci. USA., 98, 5538-5543
(16)Shibata, T., Nishinaka, T., Mikawa, T., Aihara, H., Kurumizaka, H., Yokoyama, S. and Ito, Y. (2001) "Homolorous genetic recombination as an intrinsic dynamic property of a DNA structure induced by RecA/Rad51-family proteins: A possible advantage of DNA over RNA as genomic material." Proc. Natl. Acad. Sci. USA., 98, 8425-8432.
(17)Kagawa, W., Kurumizaka, H., Ikawa, S., Yokoyama, S. and Shibata, T. (2001) "Homologous pairing promoted by the human Rad52 protein." J Biol Chem, 276, 35201-35208.
Research project
  • Core Research for Evolutional Science and Technology;Structure and Function of Genomes
Information research report
  • (In Japanese)柴田 武彦. ゲノム構造と機能 組換えを介したゲノム動態制御. 戦略的基礎研究推進事業 平成11年度 研究年報.科学技術振興事業団, 2000. p.731 - 744.