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(In Japanese)環境低負荷型の高分子物質生産システムの開発

Research report code R000000305
Posted date Sep 30, 2002
  • (In Japanese)土肥 義治
  • (In Japanese)理化学研究所
Research organization
  • (In Japanese)理化学研究所
Report name (In Japanese)環境低負荷型の高分子物質生産システムの開発
Technology summary (In Japanese)生物の物質生産機能を利用した再生可能な生物有機資源(糖や油脂など)の生産や,二酸化炭素を原料とする高分子材など有用物質生産技術の開発は「持続可能な社会」を実現する上で重要課題である。生物は生命活動として核酸,蛋白質,多糖類など多種多様な高分子物質を生産し,種々の機能を発現する生体材料そしての機能を果たした後,体内代謝や環境微生物による分解で二酸化炭素と水になり,光合成などで再び利用されて循環サイクルを形作る。これは化石燃料消費による環境問題を低減する解でもある。本研究ではこうした背景下で,主に生分解性高分子(バイオポリエステル)を対象に,生合成,機能解明,構造解析,遺伝子解析,高生産生物の育種,効率的生産,高性能材料化,リサイクル技術の開発に至る一連の基礎研究を異分野の研究者の協同作業として実施している。これらは平行して進行中であり,新規ポリエステルを合成する細菌の遺伝子解析など,一部成果は出始めている。
Research field
  • Microorganism physiology in general
  • Forest botany
  • Substance production by microorganisms, cultured tissues/cells in general
  • Relation between energy utilization and environment
  • Environmental problems
Published papers related (In Japanese)(1)T. Ohura, Y. Aoyagi, K. Takagi, Y. Yoshida, K. Kasuya and Y. Doi: Biodegradation of Poly(3-hydroxyalkanoic acids) Fibers and Isolation of Poly(3-hydroxybutyric acid)-Degrading Microorganisms under Aquatic Environments; Polym. Degrad. Stab., 63, 23-29 (1999)
(2)T. Ohura, K. Kasuya and Y. Doi: Cloning and Characterization of the Polyhydroxybutyrate Depolymerase Gene of Pseudomonas stutzeri and Analysis of the Function of Substrate-Binding Domains; Appl. Environ. Microbiol., 65, 189-197 (1999)
(3)T. Fukui, S. Yokomizo, G. Kobayashi and Y. Doi: Co-expression of Polyhydroxyalkanoate Synthase and (R)-Enoyl-CoA Hydratase Genes of Aeromonas caviae Establishes Copolyester Biosynthesis Pathway in Escherichia coli; FEMS Microbiol. Lett., 170, 69-75 (1999)
(4)Y. He, T. Masuda, A. Cao, N. Yoshie, Y. Doi and Y. Inoue: Thermal, Crystallization, and Biodegaradation Behavior of Poly(3-hydroxybutyrate) Blends with Poly(butylene succinate-co-butylene adipate) and Poly(butylene succinate-co-e-caprolactone); Polym. J., 31, 184-192 (1999)
(5)Y. Yoshie, M. Fujiwara, K. Kasuya, H. Abe, Y. Doi and Y. Inoue: Effect of Monomer Composition and Composition Distribution on Enzymatic Degradation of Poly(3-hydroxybutyrate-co-3-hydroxyvalerate); Macromol. Chem. Phys., 200, 977-982 (1999)
(6)H. Nakashita, Y. Arai, K. Yoshioka, T. Fukui, Y. Doi, R. Usami, K. Horikoshi and I. Yamaguchi: Production of Biodegradable Polyester by a Transgenic Tobacco; Biosci. Biotechnol. Biochem., 63, 870-874 (1999)
(7)K. Kasuya, T. Ohura, K. Masuda and Y. Doi: Substrate and Binding Specificities of Bacterial Polyhydroxybutyrate Depolymerases; Int. J. Biol. Macromol., 24, 329-336 (1999)
(8)T. Kichise, T. Fukui, Y. Yosida and Y. Doi: Biosynthesis of Polyhydroxyalkanoates(PHA) by Recombinant Ralstonia eutropha and Effects of PHA Synthase Activity on in vivo PHA Biosynthesis; Int. J. Biol. Macromol., 25, 69-77 (1999)
(9)K. Sudesh, T. Fukui, K. Taguchi, T. Iwata and Y. Doi: Improved Production of Poly(4-hydroxybutyrate) by Comamonas acidovorans and its Freeze-Fracture Morphology; Int. J. Biol. Macromol., 25, 79-85 (1999)
(10)S. Kusaka, T. Iwata and Y. Doi; Properties and Biodegradability of Ultra-high-molecular-wight Poly[(R)-3-hydroxybutyrate] Produced by a Recombinant Escherichia coli; Int. J. Biol. Macromol., 25, 87-94 (1999)
(11)T. Iwata, Y. Doi, S. Nakayama, H. Sasatsuki and S. Teramachi: Structure and Enzymatic Degradation of Poly(3-hydroxybutyrate) Copolymer Single Crystalswith an Extracellular PHB Depolymerase from Alcligenes faecalis T1; Int. J. Biol. Macromol., 25, 169-176 (1999)
(12)H. Abe, Y. Kikkawa, H. Aoki, T. Akehata, T. Iwata and Y. Doi: Crystallization Behavior and Thermal Properties of Melt-Crystallized Poly[(R)-3-hydroxybutyric acid-co-6-hydroxyhexanoic acid] Films; Int. J. Biol. Macromol., 25, 177-183 (1999)
(13)H. Abe and Y. Doi: Structural Effects on Enzymatic Degradabilities for Poly[(R)-3-hydroxybutyric acid] and Its Copolymers; Int. J. Biol. Macromol., 25, 185-192 (1999)
(14)H. Mitomo and Y. Doi: Lamellar Thickening and Cocrystallization of Poly(hydroxyalkanoate)s on Annealing; Int. J. Biol. Macromol., 25, 201-205 (1999)
(15)K. Taguchi, Y. Aoyagi, H. Matsusaki, T. Fukui and Y. Doi: Co-expression of 3-Ketoacyl-ACP Reductase and Polyhydroxyalkanoate Synthase Genes Induces PHA Production in Escherichia coli HB101 Strain; FEMS Microbiol. Lett., 176, 183-190 (1999)
(16)T. Iwata and Y. Doi: Enzymatic Degradation of Biosynthetic and Chemosynthetic Polymers; “Biochemical Principles and Mechanisms of Biosynthesis and Biodegradation of Polymers” Ed. by A. Steinbuhel, Wiley-VCH, 214-221 (1999)
(17)K. Tabata, K. Kasuya, H. Abe, K. Masuda and Y. Doi: Poly(aspartic acid) Degradation by a Sphingomonas sp. Isolated from Freshwater; Appl. Environ. Microbiol., 65, 4268-4270 (1999)
(18)K. Taguchi, Y. Aoyagi, H. Matsusaki, T. Fukui and Y. Doi: Over-Expression of 3-Ketoacyl-ACP Synthase III or Malonyl-CoA-ACP Transacylase Gene Induces Monomer Supply for Polyhydroxybutyrate Production in Escherichia coli HB 101; Biotech. Lett., 21, 579-584 (1999)
(19)A. Cao, Y. Arai, N. Yoshie. K. Kasuya, Y. Doi and Y. Inoue: Solid Structure and Biodegradation of the Compositionally Fractionated Poly(3-hydroxybutyric acid-co-3-hydroxypropionic acid)s; Polymer, 40, 6821-6830 (1999)
(20)T. Iwata and Y. Doi: Crystal Structure and Biodegradation of Aliphatic Polyester Crystals; Macromol. Chem. Phys., 200, 2429-2442 (1999)
(21)T. Iwata, Y. Doi, F. Kokubu and S. Teramachi: Alkaline Hydrolysis of Solution-Grown Poly[(R)-3-hydroxybutyrate] Single Crystals; Macromolecules, 32, 8325-8330 (1999)
Research project
  • Core Research for Evolutional Science and Technology;Social Systems for Better Environment Performance
Information research report
  • (In Japanese)土肥 義治. 環境低負荷型の社会システム 環境低負荷型の高分子物質生産システムの開発. 戦略的基礎研究推進事業 平成11年度 研究年報.科学技術振興事業団, 2000. p.1053 - 1057.